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Merck

AGRLE-RO

Roche

Agarose LE

low electroendosmosis

Synonym(s):

agarose

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UNSPSC Code:
12352200

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biological source

algae (seaweed)

form

powder

packaging

pkg of 100 g (11685660001)
pkg of 500 g (11685678001)

manufacturer/tradename

Roche

EEO

0.05 -0.13

transition temp

gel point 36 °C ±1.5

gel strength

>=2,500 g/cm2

foreign activity

Dnase, none detected
Rnase, none detected

shipped in

ambient

storage temp.

20-25°C

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AGRMP-ROA6013A0576
Agarose Type I, low EEO

A6013

Agarose

Agarose Low EEO

A0576

Agarose

form

powder

form

powder

form

powder

form

powder

gel strength

>=2,500 g/cm2

gel strength

-

gel strength

≥1200 g/cm2 (1% gel)

gel strength

≥1800 g/cm2 (1% gel), ≥3200 g/cm2 (1.5% gel)

EEO

0.05 -0.13

EEO

-

EEO

0.09-0.13

EEO

≤0.12

foreign activity

Dnase, none detected, Rnase, none detected

foreign activity

-

foreign activity

-

foreign activity

-

biological source

algae (seaweed)

biological source

algae (seaweed)

biological source

algae (Gelidium & Gracilaria)

biological source

-

packaging

pkg of 100 g (11685660001)

packaging

pkg of 100 g (11388983001), pkg of 500 g (11388991001)

packaging

-

packaging

-

General description

Agarose is obtained from seaweed and contains repeated agarobiose units. During agarose gel formation, the polymers aggregate to form a network with varying pore sizes. This property is used in agarose gel electrophoresis to separate DNA.[1]

Application

Agarose LE has been used for:

  • analyzing fragments between 0.2 and 15 kb
  • analysis of PCR products[2]
  • examination of restriction endonucleases
  • digests of plasmid, cosmid, and λ phage DNA
  • electrophoresis of RNA in, e.g., denaturing gels containing formaldehyde
  • in combination with ethidium bromide ​in DNA electrophoresis to facilitate the separation and visualization of DNA fragments.[3]
Nucleic acid fragments separated with Agarose LE can be blotted to nylon or nitro-cellulose membranes by all standard blotting techniques.
Important Note: Detection with nonradioactive probes e.g., digoxigenin-labeled nucleic acids, does not interfere with the use of Agarose LE.

Quality

Absence of DNase: none detected according to the current Quality Control procedures.
Absence of RNase: none detected according to the current Quality Control procedures.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

does not flash

Flash Point(C)

does not flash


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