AGRLE-RO

Roche

Agarose LE

Name: Agarose LE
Brand: ROCHE

low electroendosmosis

Synonym(s):

agarose

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About This Item

UNSPSC Code:

12352200

Key Documents

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biological source

algae (seaweed)

form

powder

packaging

pkg of 100 g (11685660001)
pkg of 500 g (11685678001)

manufacturer/tradename

Roche

EEO

0.05 -0.13

transition temp

gel point 36 °C ±1.5

gel strength

>=2,500 g/cm²

foreign activity

Dnase, none detected
Rnase, none detected

shipped in

ambient

storage temp.

20-25°C

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This Item	AGRMP-RO	A6013	A0576
AGRLE-RO Agarose LE	AGRMP-RO Agarose MP	A6013 Agarose	A0576 Agarose
form powder	form powder	form powder	form powder
gel strength >=2,500 g/cm²	gel strength -	gel strength ≥1200 g/cm² (1% gel)	gel strength ≥1800 g/cm² (1% gel), ≥3200 g/cm² (1.5% gel)
EEO 0.05 -0.13	EEO -	EEO 0.09-0.13	EEO ≤0.12
foreign activity Dnase, none detected, Rnase, none detected	foreign activity -	foreign activity -	foreign activity -
biological source algae (seaweed)	biological source algae (seaweed)	biological source algae (Gelidium & Gracilaria)	biological source -
packaging pkg of 100 g (11685660001)	packaging pkg of 100 g (11388983001), pkg of 500 g (11388991001)	packaging -	packaging -

General description

Agarose is obtained from seaweed and contains repeated agarobiose units. During agarose gel formation, the polymers aggregate to form a network with varying pore sizes. This property is used in agarose gel electrophoresis to separate DNA.[1]

Application

Agarose LE has been used for:

analyzing fragments between 0.2 and 15 kb
analysis of PCR products[2]
examination of restriction endonucleases
digests of plasmid, cosmid, and λ phage DNA
electrophoresis of RNA in, e.g., denaturing gels containing formaldehyde
in combination with ethidium bromide in DNA electrophoresis to facilitate the separation and visualization of DNA fragments.[3]

Nucleic acid fragments separated with Agarose LE can be blotted to nylon or nitro-cellulose membranes by all standard blotting techniques.
Important Note: Detection with nonradioactive probes e.g., digoxigenin-labeled nucleic acids, does not interfere with the use of Agarose LE.

Quality

Absence of DNase: none detected according to the current Quality Control procedures.
Absence of RNase: none detected according to the current Quality Control procedures.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

does not flash

Flash Point(C)

does not flash

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