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DNAPOL-RO

Roche

DNA Polymerase I

from Escherichia coli lysogenic for NM 964

Synonym(s):
kornberg polymerase
Enzyme Commission number:

Quality Level

form

solution

specific activity

8000 units/mg protein (At least 8,000 U/mg, using poly [d(A-T)] as template at 37 °C, according to Richardson (Richardson, C. C. et al., 1964, J. Biol. Chem. 239, 222).)

packaging

pkg of 1,000 U (10642720001)
pkg of 250 U (10642711001)

manufacturer/tradename

Roche

shipped in

dry ice

storage temp.

−20°C

General description

Recombinant DNA polymerase I is a DNA-dependent 5′→ 3′ polymerase that has both 5′ → 3′ and 3′ → 5′ exonuclease activities.

Specificity

Specific Activity: 8,000 U/mg
At least 8,000 U/mg, using poly [d(A-T)] as template at +37°C, according to Richardson.
Source: The enzyme is the product of the E. coli pol A gene, which has been cloned into an overproducing lysogenic strain.

Quality

Tested for the absence of endonucleases according to the current Quality Control procedures.

Unit Definition

One unit is the enzyme activity which incorporates 10 nmol of total nucleotide into an acid-precipitable fraction in 30 minutes.

Physical form

Solution, 5 U/μl in storage buffer: 50 mM potassium phosphate, 0.25 mM dithiothreitol, 50%glycerol (v/v); pH approx. 7.

Preparation Note

Activator: Mg2+

Other Notes

For life science research only. Not for use in diagnostic procedures.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

does not flash

Flash Point(C)

does not flash

Certificate of Analysis

Certificate of Origin

Y Sun et al.
Proceedings of the National Academy of Sciences of the United States of America, 98(26), 15306-15311 (2001-12-14)
Globins are oxygen-binding heme proteins present in bacteria, protists, fungi, plants, and animals. Their functions have diverged widely in evolution, and include binding, transport, scavenging, detoxification, and sensing of gases like oxygen, nitric oxide, and carbon monoxide. Neuroglobin (Ngb) is

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