The Expand High FidelityPLUS PCR System is a blend of Taq DNA Polymerase and a thermostable proofreading protein that lacks polymerase activity.
This enzyme blend is designed to amplify fragments up to 5 kb from all types of DNA with high yield and fidelity.
In addition, this enzyme blend also incorporates dUTP and, in combination with Uracil-DNA Glycosylase, can be used to safeguard PCR reactions from cross contamination.
The fidelity of DNA synthesis with the Expand High FidelityPLUS PCR System shows a 2-fold increase compared to the fidelity obtained with the Expand High Fidelity PCR System and a 6-fold increase compared to the fidelity obtained with Taq DNA Polymerase.
These characteristics make the Expand High FidelityPLUS PCR System blend the product of choice if a larger number of targets need to be amplified at the same time with high yield in combination with improved accuracy. In addition, the product can be used for the efficient labeling of DNA fragments with radioactive or non-radioactive modified nucleotides.
Use of this product is covered by US patent claims and corresponding patent claims outside the US. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patent claims require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
Expand is a trademark of Roche