Merck
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IPTG-RO

Roche

Isopropyl-β-D-thiogalactoside

mol wt 238.3, pkg of 1 g (10724815001), pkg of 5 g (11411446001)

Synonym(s):
iptg, Isopropyl β-D-thiogalactoside, Isopropyl β-D-1-thiogalactopyranoside, IPTG
Empirical Formula (Hill Notation):
C9H18O5S
CAS Number:
Molecular Weight:
238.30
Beilstein:
4631
MDL number:
PubChem Substance ID:

form

powder

mol wt

238.3

packaging

pkg of 1 g (10724815001)
pkg of 5 g (11411446001)

manufacturer/tradename

Roche

shipped in

wet ice

storage temp.

2-8°C

SMILES string

CC(C)S[C@@H]1OC(CO)[C@H](O)C(O)[C@H]1O

InChI

1S/C9H18O5S/c1-4(2)15-9-8(13)7(12)6(11)5(3-10)14-9/h4-13H,3H2,1-2H3/t5-,6+,7+,8-,9+/m1/s1

Inchi Key

BPHPUYQFMNQIOC-NXRLNHOXSA-N

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General description

Isopropyl-β-D-thiogalactoside (IPTG) is a chemical analog of galactose that cannot be cleaved by β-galactosidase. IPTG functions by binding to the lacI repressor and altering its conformation, which prevents the repression of the β-galactosidase coding gene lacZ.

Application

Isopropyl-β-D-thiogalactoside (IPTG) is commonly used in cloning procedures that require induction of β-galactosidase activity. It is used in conjunction with X-Gal or Bluo-Gal in blue-white selection of recombinant bacterial colonies that induce expression of the lac operon in Escherichia coli. It has been used for the expression of recombinant genes in E. coli.
IPTG is commonly used in cloning procedures that require induction of β-galactosidase activity. It is used in conjunction with X-Gal or Bluo-Gal in blue-white selection of recombinant bacterial colonies that induce expression of the lac operon in Escherichia coli. IPTG functions by binding to the lacI repressor and altering its conformation, which prevents the repression of the β-galactosidase coding gene lacZ.

Other Notes

Non-metabolizable galactose analog.
For life science research only. Not for use in diagnostic procedures.
The amount of labeled DNA depends on:
  • amount of template DNA
  • purity of template DNA
  • conformation of template DNA
  • average size of labeled fragment

Specifications

Assay Time: 50minutes
Specific Activity: The described standard assay will results in a specific activity of 1.8 x 109 dpm/μg, corresponding to 65% incorporation with different substrate DNAs in 30minutes. When varying the ratio of template DNA to labeled dNTP, similar incorporation rates, but different levels of specific activity of the labeled probe are obtained.

Preparation Note

Sample Material
10 ng to 3μg template DNA, linearized and at least 100 to 200bp long
Working concentration: Final concentration should be 0.2 mM.
Storage conditions (working solution): Stock solutions of the product in water (e.g., 1 M IPTG) stored at -15 to -25 °C, are stable up to six months.

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

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Certificate of Origin

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