suitable for qPCR, 2 ×, Universal


Quality Level

shelf life

≤12 mo.


kit of 1 mL (100 x 20 μL rxn; KK4600)
kit of 10 mL (1000 x 20 μL rxn; KK4602)
kit of 5 mL (500 x 20 μL rxn; KK4601)
kit of 50 mL (5000 x 20 μL rxn; KK4618)




2 ×


qPCR: suitable


for use with Agilent Mx3000P
for use with Agilent Mx3005P
for use with Agilent Mx4000
for use with Applied Biosystems® 5700
for use with Applied Biosystems® 7000
for use with Applied Biosystems® 7300
for use with Applied Biosystems® 7500
for use with Applied Biosystems® 7700
for use with Applied Biosystems® 7900HT
for use with Applied Biosystems® ViiA 7
for use with QuantStudio
for use with StepOne
for use with StepOnePlus

shipped in

dry ice

storage temp.


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General description

KAPA SYBR® FAST qPCR Kits contain the first DNA polymerase engineered via directed evolution to be more tolerant of SYBR Green I dye inhibition.

The improved robustness, processivity, and speed of KAPA SYBR FAST qPCR Kits result in consistently high amplification efficiencies enabling more accurate relative quantification for gene expression analysis. KAPA SYBR FAST qPCR Kits, developed to perform optimally in stringent real-time PCR reaction conditions, exhibit dramatic improvements to signal-to-noise ratio (fluorescence), quantification cycle (Cq), linearity, and sensitivity.


KAPA SYBR® FAST has been used in:
  • Gene expression
  • Gene knockdown validation
  • Microarray validation
  • ChIP analysis
  • Low copy detection
  • Absolute quantification of NGS libraries
  • Quantitative real-time PCR

Features and Benefits

Quantitate changes in gene expression more accurately
  • High reaction efficiency between 95 - 105% improves accuracy and reproducibility.
  • Unbiased efficiency across a wide range of GC contents and amplicon lengths.

Detect low copy and difficult targets consistently
  • Improved processivity results in earlier Cq scores.
  • Higher fluorescence detection across varying AT- and GC-rich targets.
  • Novel enzyme is resistant to SYBR® inhibition.

Complete real-time PCR runs in just 40 minutes
  • 55% shorter run times with fast cycling protocol.
  • Maintain high performance when switching from standard to fast protocols.

Quick Notes:
  • This kit contains an engineered enzyme optimized for qPCR using SYBR Green I dye chemistry.
  • The 2X master mix contains a proprietary buffer. Together with the novel enzyme, this improves amplification efficiency of both GC- and AT-rich targets.
  • 20 sec initial denaturation at 95°C is sufficient for enzyme activation. When working with complex templates, an initial denaturation of 3 min is recommended.
  • For 3-step cycling, use 20 sec for primer annealing and 1 sec for extension/data acquisition at 72°C.
  • Do not exceed 25 μL reaction volumes.


All kit components are subjected to stringent functional quality control, are free of detectable contaminating exo and endonuclease activity, and meet strict requirements with respect to DNA contamination.

Preparation Note

Always ensure that components have been fully thawed and thoroughly mixed before use. KAPA SYBR® FAST qPCR Master Mix (2X) may not freeze solidly, even when stored at -20°C.

The SYBR Green I dye contained in KAPA SYBR FAST qPCR Master Mix (2X) and ROX/fluorescein dyes (depending on kit configuration) are light sensitive. Exposure to direct light for an extended period of time will result in loss of fluorescent signal intensity.

KAPA SYBR FAST qPCR Master Mix (2X) is stable through 30 freeze-thaw cycles. Ensure that all reagents are stored protected from light at -20°C when not in use. When protected from light, reagents are stable in the dark at 4°C for at least one week and may be stored at this temperature for short-term use, provided that they do not become contaminated with microbes and/or nucleases.

Other Notes

For Research Use Only. Not for use in diagnostic procedures.

Legal Information

Applied Biosystems is a registered trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries
QuantStudio is a trademark of IROA Technologies LLC
SYBR is a registered trademark of Life Technologies
StepOne is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries
StepOnePlus is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries

Kit Components Only

Product No.

  • KAPA SYBR® FAST DNA Polymerase

  • Reaction buffer

  • dNTPs

  • SYBR® Green I dye

  • MgCl2 2.5 mM

  • ROX High Reference Dye (50X)

  • ROX Low Reference Dye (50X)

  • Stabilizers

See All (8)


Health hazard







12 - Non Combustible Liquids

WGK Germany


Flash Point F

does not flash

Flash Point C

does not flash

Certificate of Analysis

Certificate of Origin

Inhibitory effects of eugenol on RANKL-induced osteoclast formation via attenuation of NF-KB and MAPK pathways.
Deepak V, et al.
Connective tissue research, 56(3), 195-203 (2015)
Regulation of CYP11B1 and CYP11B2 steroidogenic genes by hypoxia-inducible miR-10b in H295R cells.
Nusrin S, et al.
Marine Pollution Bulletin, 85(2), 344-351 (2014)
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