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T4DNAPOL-RO

Roche

T4 DNA Polymerase

from T4 plasmid pTL43W infected Escherichia coli 71–18

Enzyme Commission number:

Quality Level

form

solution

packaging

pkg of 100 U (11004786001)
pkg of 500 U (11004794001)

manufacturer/tradename

Roche

shipped in

dry ice

storage temp.

−20°C

General description

T4 DNA Polymerase is a DNA-dependent DNA polymerase that catalyzes the addition of deoxynucleoside-5′-triphosphates to the hydroxyl termini of recessive ends.
The enzyme has a very active 3′→5′ exonuclease with a high specificity for single-stranded DNA; it lacks 5′→3′ exonuclease activity.
T4 DNA Polymerase is a DNA-dependent DNA polymerase that catalyzes the addition of deoxynucleoside-5′-triphosphates to the hydroxyl termini of recessive ends.
The enzyme has a very active 3′→5′ exonuclease with a high specificity for single-stranded DNA; it lacks 5′→3′ exonuclease activity.

Contents

  • Enzyme Solution, 1 U/μl, in buffer, pH 8.0
  • Incubation Buffer, 5x concentrated

Application

  • Sample Materials: DNA with 5′-protruding ends
Site-directed mutagenesis: Together with T4 gene 32 protein, T4 DNA polymerase can fill in single-stranded gaps during site-directed mutagenesis experiments.
  • Superiority of hybridization probes prepared with T4 DNA polymerase: Polymerase-labeled probes have two advantages over probes prepared by nick translation:
a) They do not have artificial hairpin structures.
b) They can easily be converted into strand-specific probes by restriction endonuclease cleavage.
T4 DNA polymerase is used to label 3′-termini of DNA. Extensive labeling is achieved by the replacement reaction, in which the 3′-exonuclease activity of the enzyme first digests dsDNA to produce molecules with recessed 3′-termini. On subsequent addition of labeled dNTPs, the polymerase activity of T4 DNA polymerase then extends the 3′ ends along the length of the template. Exonuclease III from E. coli can be used to create partially single-stranded dsDNA for subsequent polymerization reactions. Molecules labeled to high specific activity are used primarily as hybridization probes. They have two advantages over probes prepared by nick translation: they lack artificial hairpin structures and they can easily be converted into strand-specific probes by cleavage with suitable restriction endonucleases. In combination with T4 Gene 32 Protein, T4 DNA polymerase is used for gap-filling in site-directed mutagenesis experiments.

Packaging

1 kit containing 2 components

Quality

Absence of endonucleases, ss-specific DNases, nicking activity, and RNases tested according to the current Quality Control procedures.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Kit Components Only

Product No.
Description

  • Enzyme Solution, in buffer, pH 8.0 1 U/μl

  • Incubation Buffer 5x concentrated

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

does not flash

Flash Point(C)

does not flash

Certificate of Analysis

Certificate of Origin

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