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Taq DNA Polymerase, 1 U/μl

suitable for PCR, hotstart: no, dNTPs included: no

polymerase, primer extension, dna amplification, pcr
Enzyme Commission number:

Quality Level


sufficient for ≤2,000 reactions (11647687001)
sufficient for ≤500 reactions (11647679001)


dNTPs included: no
hotstart: no


pkg of 1,000 U (11647687001 [4 x 250 U])
pkg of 250 U (11647679001)




72 °C optimum reaction temp.


PCR: suitable


purified DNA

optimum pH

~9.0 (20 °C)

shipped in

dry ice

storage temp.


General description

The enzyme was cloned in E.coli and is isolated to be free of unspecific endo- or exonucleases according to the current quality control procedurese. Taq DNA Polymerase is a highly processive 5′–3′ DNA polymerase that lacks 3′–5′ exonuclease activity. It consists of a single polypeptide chain with a molecular weight of approximately 95 kDa. The enzyme exhibits highest activity at a pH of around 9 (adjusted at 20 °C) and temperatures around +75 °C. Taq DNA Polymerase also accepts modified deoxyribonucleosidetriphosphates as substrates, and can be used to label DNA-fragments either with radionucleotides, digoxigenin, fluorescein or biotin.
The high processivity, absence of exonuclease activity and temperature optima of Taq DNA Polymerase enable the use of this enzyme in DNA sequencing, especially where the resolution of secondary structures plays a major role.


This lower concentration of our recombinant Taq DNA Polymerase allows small amounts of the polymerase to be pipetted more accurately and conveniently. In all other respects, this preparation is identical to our higher concentration (5 U/μl) preparation and can be used for:
  • PCR
  • RT-PCR
  • Other primer-extension reactions, such as sequencing and labeling


1 kit containing 4 components

Unit Definition

One unit Taq DNA Polymerase is defined as the amount of enzyme that incorporates 10 nmol of total deoxyribonucleoside triphosphates into acid precipitable DNA within 60 min at +65 °C under the assay conditions stated above.

Unit Assay: Incubation buffer:
67 mM Tris/HCl; pH 8.3/25 °C, 5 mM MgCl2, 10 mM Mercaptoethanol, 0.2% Polydocanol, 0.2 mg/ml Gelatine, 0.2 mM each dATP, dGTP, dTTP and 0.1 mM dCTP.

Incubation procedure:
M13mp9ss, M13 primer (17mer) and 1 μCi (α-32P) dCTP are incubated with suitable dilutions of Taq DNA Polymerase in 50 μl incubation buffer at +65 °C for 60 minutes. The amount of incorporated dNTPs is determined by trichloroacetic acid precipitation.

Volume Activity: 1 U/μl

Other Notes

For life science research only. Not for use in diagnostic procedures.

Legal Information

Use of this product is covered by US patent claims and corresponding patent claims outside the US. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patent claims require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.

Kit Components Only

Product No.

  • Taq DNA Polymerase 1 U/μl

  • PCR Buffer with MgCl<sub>2</sub> 10x concentrated

  • MgCl<sub>2</sub> Stock Solution

  • PCR Buffer without MgCl<sub>2</sub>

Hazard Statements

Precautionary Statements

Hazard Classifications

Aquatic Chronic 3

Storage Class Code

12 - Non Combustible Liquids



Flash Point(F)

does not flash

Flash Point(C)

does not flash

Certificate of Analysis

Certificate of Origin

Christopher E Carr et al.
Astrobiology, 13(1), 68-78 (2013-01-22)
Life on Mars, if it exists, may share a common ancestry with life on Earth derived from meteoritic transfer of microbes between the planets. One means to test this hypothesis is to isolate, detect, and sequence nucleic acids in situ
Rupali Srivastava et al.
Stem cells (Dayton, Ohio), 31(4), 652-665 (2012-12-12)
Directing differentiation of embryonic stem cells (ESCs) to specific neuronal subtype is critical for modeling disease pathology in vitro. An attractive means of action would be to combine regulatory differentiation factors and extrinsic inductive signals added to the culture medium.
Riccardo Sangermano et al.
Ophthalmology, 123(6), 1375-1385 (2016-03-16)
To elucidate the functional effect of the ABCA4 variant c.5461-10T→C, one of the most frequent variants associated with Stargardt disease (STGD1). Case series. Seventeen persons with STGD1 carrying ABCA4 variants and 1 control participant. Haplotype analysis of 4 homozygotes and
Hailun Ma et al.
Journal of virology, 85(13), 6579-6588 (2011-05-06)
Endogenous retroviral sequences are present in high copy numbers in the genomes of all species and may be expressed as RNAs; however, the majority are defective for virus production. Although virus has been isolated from various Old World monkey and

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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