All Photos(1)

TAQNL-RO

Roche

Taq DNA Polymerase (1 U/μl), dNTPack

suitable for PCR, optimum pH ~9.0 (20 °C)

Synonym(s):
Taq | PCR | DNA amplification
Enzyme Commission number:

Quality Level

usage

sufficient for ≤2,000 reactions (04738241001)
sufficient for ≤500 reactions (04738225001)

feature

dNTPs included
hotstart: no

packaging

pkg of 1,000 U (04738241001 [4 x 250 U])
pkg of 250 U (04738225001)

manufacturer/tradename

Roche

parameter

72 °C optimum reaction temp.

technique(s)

PCR: suitable

input

purified DNA

optimum pH

~9.0 (20 °C)

shipped in

dry ice

storage temp.

−20°C

General description

Taq DNA Polymerase (1 U/μl), dNTPack, comprises Taq DNA Polymerase and a ready-to-use solution of PCR grade nucleotides. Taq DNA polymerase is a highly processive 5′-3′ DNA polymerase that lacks 3′–5′ exonuclease activity. It is stable during prolonged incubations at elevated temperatures (+95 °C). Additionally, it exhibits highest activity at a pH of around 9 (adjusted at 20 °C) and temperatures around +75 °C. It accepts dNTP analogs as substrates. There is no difference in stability or performance when compared to the standard concentration of Taq DNA Polymerase.

Application

Taq DNA Polymerase (1 U/μl), dNTPack may be used for:
  • PCR
  • RT-PCR
  • Other primer-extension reactions, such as sequencing and labeling

Packaging

1 kit containing 5 components

Unit Definition

One unit Taq DNA Polymerase is defined as the amount of enzyme that incorporates 10 nmol of total deoxyribonucleosidetriphosphates into acid precipitable DNA within 30 minutes at +75 °C under the assay conditions stated under unit assay.

Unit Assay: Incubation buffer:
67 mM Tris/HCl; pH 8.3/25 °C, 5 mM MgCl2, 10 mM Mercaptoethanol, 0.2% Polydocanol, 0.2 mg/ml Gelatine, 0.2 mM each dATP, dGTP, dTTP and 0.1 mM dCTP.

Incubation procedure:
M13mp9ss, M13 primer (17mer) and 1 μCi (α-32P) dCTP are incubated with suitable dilutions of Taq DNA Polymerase in 50 μl incubation buffer at +65 °C for 60 minutes. The amount of incorporated dNTPs is determined by trichloroacetic acid precipitation.

Volume Activity: 1 U/μl

Other Notes

For life science research only. Not for use in diagnostic procedures.

Kit Components Only

Product No.
Description

  • Taq DNA Polymerase

  • PCR Buffer with MgCl<sub>2</sub> 10x concentrated

  • MgCl<sub>2</sub> Stock Solution

  • PCR Buffer without MgCl<sub>2</sub>

  • PCR Nucleotide Mix</ul>

Hazard Statements

Precautionary Statements

Hazard Classifications

Aquatic Chronic 3

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

does not flash

Flash Point(C)

does not flash

Certificate of Analysis

Certificate of Origin

Random mutagenesis of gene-sized DNA molecules by use of PCR with Taq DNA polymerase.
Y H Zhou et al.
Nucleic acids research, 19(21), 6052-6052 (1991-11-11)

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service