365548

Direct Red 80

Dye content 25 %

• Synonym(s): Sirius Red
• Empirical Formula (Hill Notation): C₄₅H₂₆N₁₀Na₆O₂₁S₆
• CAS Number: 2610-10-8
• Molecular Weight: 1373.07
• Colour Index Number: 35780
• Beilstein: 8185575
• EC Number: 220-027-3
• MDL number: MFCD00054389
• PubChem Substance ID: 24862536
• NACRES: NA.47

Properties

• form: powder
• Quality Level: 200
• composition: Dye content, 25%
• λmax: 528 nm
• ε (extinction coefficient): ≥12000 at 277-283 nm in H₂O; ≥15000 at 223-235 nm in H₂O; ≥32000 at 524-530 nm in H₂O
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• SMILES string: [Na+].[Na+].[Na+].[Na+].[Na+].[Na+].Oc1c(\N=N\c2ccc(cc2S([O-])(=O)=O)\N=N\c3ccc(cc3)S([O-])(=O)=O)c(cc4cc(NC(=O)Nc5ccc6c(O)c(\N=N\c7ccc(cc7S([O-])(=O)=O)\N=N\c8ccc(cc8)S([O-])(=O)=O)c(cc6c5)S([O-])(=O)=O)ccc14)S([O-])(=O)=O
• InChI: 1S/C45H32N10O21S6.6Na/c56-43-33-13-5-27(17-23(33)19-39(81(71,72)73)41(43)54-52-35-15-7-29(21-37(35)79(65,66)67)50-48-25-1-9-31(10-2-25)77(59,60)61)46-45(58)47-28-6-14-34-24(18-28)20-40(82(74,75)76)42(44(34)57)55-53-36-16-8-30(22-38(36)80(68,69)70)51-49-26-3-11-32(12-4-26)78(62,63)64;;;;;;/h1-22,56-57H,(H2,46,47,58)(H,59,60,61)(H,62,63,64)(H,65,66,67)(H,68,69,70)(H,71,72,73)(H,74,75,76);;;;;;/q;6*+1/p-6/b50-48+,51-49+,54-52+,55-53+;;;;;;
• InChI key: YIQKLZYTHXTDDT-IJPLDVMMSA-H

Description

General description

Direct Red 80 (C.I. 35780) is an extremely hydrophilic, very large tetrakisazo hexasulfonated anionic dye that has a large, planar aromatic system.It is also known as Sirius red F3B, chlorantine fast red 7BLN or 5BRL, durazol brilliant red B, pontamine fast red 7BNL, and sirius fast red.

Application

Direct Red 80 has been used as an ingredient in the preparation of saturated picric acid for immunohistochemical detection and quantitation of collagen in liver tissues.Other applications include -

• detection of amyloid
• Sirius red-Van Gieson methods for quantitation of collagen using image analysis methods
• counterstaining applications substituting eosin in alum hematoxylins

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves