Merck
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62970

Sigma-Aldrich

Lysozyme from chicken egg white

dialyzed, lyophilized, powder, ~100000 U/mg

Synonym(s):
Mucopeptide N-acetylmuramoylhydrolase, Muramidase
CAS Number:
Enzyme Commission number:
EC Number:
MDL number:
NACRES:
NA.54

form

powder

Quality Level

quality

dialyzed
lyophilized

specific activity

~100000 U/mg

mol wt

single-chain 14.3 kDa
Mr ~14600

UniProt accession no.

storage temp.

2-8°C

Gene Information

chicken ... LYZ(396218)

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Application

Lysozyme from chicken egg white has been used:
  • as a standard in small-angle neutron scattering experiments and infrared absorbance spectroscopy
  • as a constituent of cell lysis buffer
  • in protein crystallization experiments

Enzyme breaks down the cell walls of bacteria; used to prepare spheroplasts.

Biochem/physiol Actions

Lysozyme hydrolyzes β(1→4) linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrin. Gram-positive cells are quite susceptible to this hydrolysis as their cell walls have a high proportion of peptidoglycan. Gram-negative bacteria are less susceptible due to the presence of an outer membrane and a lower proportion of peptidoglycan. However, these cells may be hydrolyzed in the presence of EDTA that chelates metal ions in the outer bacterial membrane.

The enzyme is active over a broad pH range (6.0 to 9.0). At pH 6.2, maximal activity is observed over a wider range of ionic strengths (0.02 to 0.100 M) than at pH 9.2 (0.01 to 0.06 M).

Unit Definition

1 U corresponds to the amount of enzyme which decreases the absorbance at 450 nm by 0.001 per minute at pH 7.0 and 25°C (Micrococcus luteus, ATCC 4698, as substrate)

Other Notes

Suitable for the hydrolysis of bacterial cell walls, mucopolysaccharides, mucopolypeptides or chitin; degradability of L. lactis subsp. cremoris H2 cell walls; Review

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Precautionary Statements

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

More documents

Quotes and Ordering

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

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    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

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Structures and interactions among lysozyme proteins below the isoelectric point in presence of divalent ions
Pandit S, et al.
Chemical Physics Letters, 711, 8-14 (2018)
L. Stevens
Comp. Biochem. Physiol., B: Comp. Biochem., 100, 1-1 (1991)
POTRA Domains, Extracellular Lid, and Membrane Composition Modulate the Conformational Stability of the beta Barrel Assembly Factor BamA
Thoma J, et al.
Structure, 26(7), 987-996 (2018)
Use of Protein Thin Film Organized by External Electric Field as a Template for Protein Crystallization
Walter TK, et al.
ACS Omega, 3(8), 8683-8690 (2018)
R.W. Franck
Bioorganic Chemistry, 20, 77-77 (1992)

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