69947

MOPS

BioUltra, for molecular biology, ≥99.5% (T)

• Synonym(s): 4-Morpholinepropanesulfonic acid, 3-(N-Morpholino)propanesulfonic acid
• Empirical Formula (Hill Notation): C₇H₁₅NO₄S
• CAS Number: 1132-61-2
• Molecular Weight: 209.26
• Beilstein: 1106776
• EC Number: 214-478-5
• MDL number: MFCD00006183
• PubChem Substance ID: 329762136
• NACRES: NA.25

Properties

• Quality Level: 200
• grade: for molecular biology
• product line: BioUltra
• assay: ≥99.5% (T)
• form: powder or crystals
• impurities: DNases, none detected; RNases, none detected; insoluble matter, passes filter test; phosphatases, none detected; proteases, none detected
• ign. residue: ≤0.1% (as SO₄)
• loss: ≤1% loss on drying, 110 °C
• pH: 2.5-4.0 (25 °C, 1 M in H₂O)
• useful pH range: 6.5-7.9
• pKa (25 °C): 7.2
• solubility: H₂O: 1 M at 20 °C, clear, colorless
• anion traces: chloride (Cl^-): ≤50 mg/kg; sulfate (SO₄^2-): ≤50 mg/kg
• cation traces: Al: ≤5 mg/kg; As: ≤0.1 mg/kg; Ba: ≤5 mg/kg; Bi: ≤5 mg/kg; Ca: ≤10 mg/kg; Cd: ≤5 mg/kg; Co: ≤5 mg/kg; Cr: ≤5 mg/kg; Cu: ≤5 mg/kg; Fe: ≤5 mg/kg; K: ≤50 mg/kg; Li: ≤5 mg/kg; Mg: ≤5 mg/kg; Mn: ≤5 mg/kg; Mo: ≤5 mg/kg; Na: ≤50 mg/kg; Ni: ≤5 mg/kg; Pb: ≤5 mg/kg; Sr: ≤5 mg/kg; Zn: ≤5 mg/kg
• λ: 1 M in H₂O
• UV absorption: λ: 260 nm A_max: 0.020; λ: 280 nm A_max: 0.015
• SMILES string: OS(=O)(=O)CCCN1CCOCC1
• InChI: 1S/C7H15NO4S/c9-13(10,11)7-1-2-8-3-5-12-6-4-8/h1-7H2,(H,9,10,11)
• InChI key: DVLFYONBTKHTER-UHFFFAOYSA-N

Description

General description

3-(N-Morpholino)propanesulfonic acid or MOPS due to its inert nature is a preferred and widely used buffer in many biochemical studies.

Application

MOPS has been used:

• as a component of Roswell Park Memorial Institute (RPMI) medium for diluting fungal inoculum
• as a buffer in capillary-zone electrophoresis to test performance
• for the dilution of proteins from algal samples

Other Notes

Easily compare specifications for MOPS products with the MOPS specification table.

Component of running buffer in the fractionation of RNA on formaldehyde agarose gels

Safety Information

• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 1
• Flash Point(F): 230.0 °F - closed cup
• Flash Point(C): 110 °C - closed cup
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves