Merck
All Photos(1)

69947

Sigma-Aldrich

MOPS

BioUltra, for molecular biology, ≥99.5% (T)

Synonym(s):
4-Morpholinepropanesulfonic acid, 3-(N-Morpholino)propanesulfonic acid
Empirical Formula (Hill Notation):
C7H15NO4S
CAS Number:
Molecular Weight:
209.26
Beilstein:
1106776
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.25

Quality Level

grade

for molecular biology

product line

BioUltra

assay

≥99.5% (T)

form

powder or crystals

impurities

DNases, none detected
RNases, none detected
insoluble matter, passes filter test
phosphatases, none detected
proteases, none detected

ign. residue

≤0.1% (as SO4)

loss

≤1% loss on drying, 110 °C

pH

2.5-4.0 (25 °C, 1 M in H2O)

useful pH range

6.5-7.9

pKa (25 °C)

7.2

solubility

H2O: 1 M at 20 °C, clear, colorless

anion traces

chloride (Cl-): ≤50 mg/kg
sulfate (SO42-): ≤50 mg/kg

cation traces

Al: ≤5 mg/kg
As: ≤0.1 mg/kg
Ba: ≤5 mg/kg
Bi: ≤5 mg/kg
Ca: ≤10 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤5 mg/kg
Cu: ≤5 mg/kg
Fe: ≤5 mg/kg
K: ≤50 mg/kg
Li: ≤5 mg/kg
Mg: ≤5 mg/kg
Mn: ≤5 mg/kg
Mo: ≤5 mg/kg
Na: ≤50 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Sr: ≤5 mg/kg
Zn: ≤5 mg/kg

λ

1 M in H2O

UV absorption

λ: 260 nm Amax: 0.020
λ: 280 nm Amax: 0.015

SMILES string

OS(=O)(=O)CCCN1CCOCC1

InChI

1S/C7H15NO4S/c9-13(10,11)7-1-2-8-3-5-12-6-4-8/h1-7H2,(H,9,10,11)

InChI key

DVLFYONBTKHTER-UHFFFAOYSA-N

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General description

3-(N-Morpholino)propanesulfonic acid or MOPS due to its inert nature is a preferred and widely used buffer in many biochemical studies.

Application

MOPS has been used:
  • as a component of Roswell Park Memorial Institute (RPMI) medium for diluting fungal inoculum
  • as a buffer in capillary-zone electrophoresis to test performance
  • for the dilution of proteins from algal samples

Other Notes

Easily compare specifications for MOPS products with the MOPS specification table.
Component of running buffer in the fractionation of RNA on formaldehyde agarose gels

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 1

Flash Point(F)

230.0 °F - closed cup

Flash Point(C)

110 °C - closed cup

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

R.C. Ogden et al.
Methods in Enzymology, 152, 82-82 (1987)
Interactions of biological buffers with macromolecules: the ubiquitous ?smart? polymer PNIPAM and the biological buffers MES, MOPS, and MOPSO
Taha M, et al.
Macromolecules, 44(21) (44)
Separation and Detection of Phosphorylated and Nonphosphorylated BvgA, a Bordetella pertussis Response Regulator, in vivo and in vitro
Chen Q, et al.
Bio-protocol, 3 (2013)
Laminarin quantification in microalgae with enzymes from marine microbes
Becker S and Hehemann JH
Bio-protocol, 8(8) (8)
Anita V Thomae et al.
Biophysical journal, 89(3), 1802-1811 (2005-06-14)
According to the pH-partition hypothesis the charged species of organic compounds do not contribute to lipid bilayer permeation as they generally show negligible partitioning into n-octanol. With this assumption, membrane permeation is related to the molar fraction of the neutral

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