Skim Milk powder is used for the demonstration of coagulation and proteolysis of casein. Skim Milk is sometimes used as a complete medium or as an ingredient in other media used for the propagation of organisms occurring in milk products. The addition of skim milk to any nutrient-rich medium creates favorable conditions for the growth of organisms, which are encountered in milk. The number of bacteria isolated thus is more than the number of organisms isolated on a regular medium. Proteolytic bacteria hydrolyze casein to form soluble nitrogenous compounds indicated as a clear zone surrounding the colonies on the agar medium. Skim milk serves as a good source of casein
Skim Milk Powder is recommended for cultivation and enumeration of microorganisms encountered in dairy industry
Mix the powder to a smooth paste with a small quantity of distilled water, then gradually add more distilled water until a 10% w/v mixture is obtained. This is equivalent to fresh milk, and may be sterilized by autoclaving for 5 minutes at 121 °C. Care should be taken not to overheat during sterilization, otherwise caramelization will occur.
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