All Photos(1)



Hydrogen chloride - 1-butanol solution

~3 M in 1-butanol, for GC derivatization, LiChropur

CAS Number:
MDL number:
PubChem Substance ID:

Quality Level


for GC derivatization



reaction suitability

reagent type: derivatization reagent
reaction type: Acylations


~3 M in 1-butanol


gas chromatography (GC): suitable


0.87 g/mL at 20 °C

storage temp.


SMILES string




InChI key


General description

Acylation, an alternative to silylation, is the conversion of compounds that contain active hydrogens (-OH, -SH and -NH) into esters, thioesters, and amides through the action of a carboxylic acid or derivative. The presence of a carbonyl group adjacent to the halogenated carbons enhances electron capture detector (ECD) response. Acylation has many benefits:
  • It improves the stability of compounds by protecting unstable groups.
  • It may confer volatility on substances such as carbohydrates or amino acids, which have so many polar groups that they are non-volatile and normally decompose on heating.
  • It assists in separations not possible with underivatized compounds.
  • Compounds are detectable at very low levels with an ECD.

Hydrogen chloride-1-butanol is a reagent utilized to form fragmentation-directing derivatives for GC/MS analysis.


Hydrogen chloride - 1-butanol solution has been used for butylation of amino acids during blood sample analysis for determination of ethylenediaminetetraacetic acid using mass spectrometry.

Other Notes

Reagent for the esterification of amino acids and carnites for GC-MS analysis

Legal Information

LiChropur is a trademark of Merck KGaA, Darmstadt, Germany

Signal Word


Hazard Classifications

Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Skin Irrit. 2

Storage Class Code

3 - Flammable liquids



Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

Certificate of Origin

M S Rashed et al.
Clinical chemistry, 43(7), 1129-1141 (1997-07-01)
Metabolic profiling of amino acids and acylcarnitines from blood spots by automated electrospray tandem mass spectrometry (ESI-MS/MS) is a powerful diagnostic tool for inborn errors of metabolism. New approaches to sample preparation and data interpretation have helped establish the methodology
Q Miao et al.
Physical review letters, 109(23), 233905-233905 (2013-02-02)
X-ray lasing is predicted to ensue when molecules are pumped into dissociative core-excited states by a free-electron-laser pulse. The lasing is due to the population inversion created in the neutral dissociation product, and the process features self-trapping of the x-ray
J Marwaha et al.
Experimental parasitology, 134(1), 12-17 (2013-02-16)
Intestinal cestodes with complex life cycles have to pass through the acid stomach lumen of their vertebrate host(s) in order to reach their preferred site of development. The cestode's tegument is the only organ in constant contact with this hostile
Woo Seok Yang et al.
Journal of ethnopharmacology, 146(2), 637-644 (2013-02-16)
Archidendron clypearia Jack. (Fabaceae) is a representative ethnomedicinal herbal plant prescribed for various inflammatory diseases such as pharyngolaryngitis and tonsillitis. However, the pharmacology behind this plant's anti-inflammatory properties has not been fully understood. Therefore, in this study, the anti-inflammatory mechanism
William J Bulsiewicz et al.
Digestive diseases and sciences, 58(7), 1955-1959 (2013-05-08)
Acid-sensing ion channels (ASICs) are esophageal nociceptors that are candidates to mediate heartburn in non-erosive reflux disease (NERD). Amiloride, a diuretic, is known to inhibit ASICs. For this reason, we sought a role for ASICs in mediating heartburn by determining

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service