88597

Hydrogen peroxide solution

3%, suitable for microbiology

• Synonym(s): Catalase Test
• Empirical Formula (Hill Notation): H₂O₂
• CAS Number: 7722-84-1
• Molecular Weight: 34.01
• Beilstein: 3587191
• MDL number: MFCD00011333
• PubChem Substance ID: 329769664
• NACRES: NA.85

Properties

• Agency: according to GB 4789.30-2016; according to ISO 10272-1:2017; according to ISO 10272-2:2017; according to ISO 11290-1:2017; according to ISO 11290-2:2017
• Quality Level: 200
• vapor pressure: 23.3 mmHg ( 30 °C)
• Assay: 2.8-3.2% (redox titration)
• form: solution
• shelf life: limited shelf life, expiry date on the label
• concentration: 1-5%
• technique(s): microbe id | specific enzyme detection: suitable
• mp: 0.0 °C
• density: 1.000 g/cm³
• application(s): clinical testing; environmental; food and beverages; veterinary; microbiology
• suitability: Pneumococcus spp.; Staphylococcus spp.; bacteria
• SMILES string: OO
• InChI: 1S/H2O2/c1-2/h1-2H
• InChI key: MHAJPDPJQMAIIY-UHFFFAOYSA-N

Description

General description

A reagent to detect the enzymes catalase and peroxidase. Catalase and peroxidase is found in most aerobe bacteria. They catalyse the breakdown of hydrogem peroxide with the release of free oxygen (resulting in bubbles).The classical test for differentiating staphylococci (positive) from streptococci (negative).To observe the action of the enzymes, catalase reagent is added to a pure bacterial culture. Any immediate bubbling is indicative of a positive result since oxygen is a by-product of hydrogen peroxide decomposition. The test is also useful in differentiating Gram positive bacilli.

Application

Hydrogen peroxide solution has been used:

• to stimulate Src homology region 2 domain-containing phosphatase-1 (SHP-1) oxidation in thymocytes
• for the induction of apoptosis in cultured human microvascular endothelial cells
• as a substrate for superoxide dismutase

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves