95290

Methyl Blue

for microscopy (Bot., Hist.), indicator (pH 9.4-14.0)

• Synonym(s): Acid blue 93, Aniline blue water soluble, Cotton blue, Poirriers blue, Water blue
• Empirical Formula (Hill Notation): C₃₇H₂₇N₃Na₂O₉S₃
• CAS Number: 28983-56-4
• Molecular Weight: 799.80
• Colour Index Number: 42780
• EC Number: 249-352-9
• MDL number: MFCD00058509
• PubChem Substance ID: 329770433
• NACRES: MA.02

Properties

• grade: for microscopy (Bot., Hist.); indicator (pH 9.4-14.0)
• Quality Level: 200
• form: powder or crystals
• pH: 9.4-14
• solubility: water: 0.01 g/10 mL, blue to very deep blue
• εmax: ≥200 at 310-320 nm; ≥400 at 595-605 nm
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• SMILES string: [Na+].[Na+].OS(=O)(=O)c1ccc(Nc2ccc(cc2)\C(c3ccc(Nc4ccc(cc4)S([O-])(=O)=O)cc3)=C5/C=CC(\C=C5)=N/c6ccc(cc6)S([O-])(=O)=O)cc1
• InChI: 1S/C37H29N3O9S3.2Na/c41-50(42,43)34-19-13-31(14-20-34)38-28-7-1-25(2-8-28)37(26-3-9-29(10-4-26)39-32-15-21-35(22-16-32)51(44,45)46)27-5-11-30(12-6-27)40-33-17-23-36(24-18-33)52(47,48)49;;/h1-24,38-39H,(H,41,42,43)(H,44,45,46)(H,47,48,49);;/q;2*+1/p-2
• InChI key: MCPLVIGCWWTHFH-UHFFFAOYSA-L

Description

General description

Methyl Blue (C.l. 42780) is a triaminotriphenylmethane dye with each amino group carrying a monosulfonated phenyl substituent. It is also known as Water blue, Acid blue 93, Cotton blue, Poirriers blue, and Aniline blue water soluble.

Application

Methyl Blue has been used:

• to stain leaf disks for callose visualization within the cells
• to incubate cells for 4′,6-diamidino-2-phenylindole (DAPI)/septa staining
• as a marker for β-(1-3)-d-glucans or acidic phloroglucinol for the detection of callose and lignin
• in Mann′s stain as it involves a methyl blue-eosin mixture

Safety Information

• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 2
• Flash Point(F): 572.0 °F - Pensky-Martens closed cup
• Flash Point(C): > 300 °C - Pensky-Martens closed cup
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves