A8456

4-(2-Aminoethyl)benzenesulfonyl fluoride hydrochloride

≥97.0% (HPLC)

• Synonym(s): AEBSF
• Empirical Formula (Hill Notation): C₈H₁₀FNO₂S · HCl
• CAS Number: 30827-99-7
• Molecular Weight: 239.69
• Beilstein: 7604627
• MDL number: MFCD00132962
• PubChem Substance ID: 24278240
• NACRES: NA.77

Properties

• biological source: synthetic (organic)
• Quality Level: 300
• assay: ≥97.0% (HPLC)
• form: powder
• mp: 183-191 °C
• solubility: H₂O: 50 mg/mL (stable for up to six months if stored refrigerated at a pH of less than 7. If a pH of greater than 7 is required, pH adjustment should be made just prior to use.)
• storage temp.: −20°C
• SMILES string: FS(C1=CC=C(CCN)C=C1)(=O)=O.[H]Cl
• InChI: 1S/C8H10FNO2S.ClH/c9-13(11,12)8-3-1-7(2-4-8)5-6-10;/h1-4H,5-6,10H2;1H
• InChI key: WRDABNWSWOHGMS-UHFFFAOYSA-N

Description

Application

4-(2-Aminoethyl)benzenesulfonyl fluoride hydrochloride (AEBSF) has been used:

• as a protease inhibitor in plasma samples to prevent acylated ghrelin (AG) degradation
• as a snake venom serine proteinase (SVSP) inhibitor in SVSP inhibition or AEBSF assay to test the involvement of SVSPs on the fibrinogen-clotting activity
• as a component in NP-40 lysis buffer to resuspend cell pellets for preparing cell lysates

Recommended concentrations for use are 0.1-1 mM.

Packaging

25, 100, 500 mg in poly bottle

Biochem/physiol Actions

AEBSF can inhibit acetylcholinesterase (AChE).

4-(2-Aminoethyl)benzenesulfonyl fluoride hydrochloride (AEBSF) is an irreversible serine protease inhibitor. Inhibition constants are similar to those of phenylmethanesulfonyl fluoride (PMSF) and diisopropylfluorophosphate (DFP). AEBSF has been shown to inhibit trypsin, chymotrypsin, plasmin kallikrein, and thrombin. As an alternative to PMSF and DFP, AEBSF offers lower toxicity, improved solubility in water, and improved stability in aqueous solutions. AEBSF can be used in cell culture in concentrations of up to 0.25 mM.

Safety Information

• Pictograms: GHS07
• Signal Word: Warning
• Hazard Statements: H315 - H319
• Precautionary Statements: P264 - P280 - P302 + P352 - P305 + P351 + P338 - P332 + P313 - P337 + P313
• Hazard Classifications: Eye Irrit. 2 - Skin Irrit. 2
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable