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A0169

Sigma-Aldrich

Agarose

Type I-A, low EEO

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Synonym(s):
3,6-Anhydro-α-L-galacto-β-D-galactan, Agarose LE
CAS Number:
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.25

Quality Level

type

Type I-A

form

powder

impurities

≤7% water

ash

≤0.5%

turbidity

≤4 NTU (1.5% gel)

EEO

0.09-0.13

mp

86.5-89.5  °C

transition temp

gel point 36 °C ±1.5 °C (1.5% gel)

gel strength

≥1200 g/cm2 (1% gel)

anion traces

sulfate (SO42-): ≤0.20%

InChI

1S/C24H38O19/c25-1-5-9(27)11(29)12(30)22(38-5)41-17-8-4-36-20(17)15(33)24(40-8)43-18-10(28)6(2-26)39-23(14(18)32)42-16-7-3-35-19(16)13(31)21(34)37-7/h5-34H,1-4H2/t5-,6-,7+,8+,9+,10+,11+,12-,13+,14-,15+,16-,17-,18+,19+,20+,21-,22+,23+,24+/m1/s1

InChI key

MJQHZNBUODTQTK-WKGBVCLCSA-N

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1 of 4

This Item
A6877A6013A4718
vibrant-m

A0169

Agarose

vibrant-m

A6877

Agarose

vibrant-m

A6013

Agarose

vibrant-m

A4718

Agarose

form

powder

form

powder

form

powder

form

powder

gel strength

≥1200 g/cm2 (1% gel)

gel strength

≥1000 g/cm2 (1.0% gel)

gel strength

≥1200 g/cm2 (1% gel)

gel strength

-

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

transition temp

gel point 36 °C ±1.5 °C (1.5% gel)

transition temp

congealing temperature 34.5-37.5 °C (1.5% gel)

transition temp

gel point 36 °C ±1.5 °C (1.5% gel)

transition temp

gel point ≤35 °C (3% gel)

impurities

≤7% water

impurities

≤10% moisture content

impurities

≤10% water

impurities

-

General description

Agarose, a natural polymer is obtained from sea algae. This neutral linear polysaccharide is made up of d-galactose and 3,6-anhydro-l-galactose. These are connected alternatively with the help of glycosidic bonds. Agarose has high significance in gel electrophoresis and gel chromatography.

Application

Agarose has been used:
  • as a component in the preparation of tumor tissue to embed the cell material
  • in the sample preparation for quantum dot analysis
  • in the preparation of agarose-solidified medium to study the effect of medium solidification on the culture of isolated ovules

Biochem/physiol Actions

Agarose is used as bioink, because of its characteristic properties such as, biocompatibility, mechanical strength and gelling ability at low temperature.

Analysis Note

The following is a list of properties associated with our agaroses:
Sulfate content - used as an indicator of purity, since sulfate is the major ionic group present.
Gel strength - the force that must be applied to a gel to cause it to fracture.
Gel point - the temperature at which an aqueous agarose solution forms a gel as it cools. Agarose solutions exhibit hysteresis in the liquid-to-gel transition - that is, their gel point is not the same as their melting temperature.
Electroendosmosis (EEO) - a movement of liquid through the gel. Anionic groups in an agarose gel are affixed to the matrix and cannot move, but dissociable counter cations can migrate toward the cathode in the matrix, giving rise to EEO. Since electrophoretic movement of biopolymers is usually toward the anode, EEO can disrupt separations because of internal convection.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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