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A6877

Sigma-Aldrich

Agarose

Medium EEO, for molecular biology

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Synonym(s):
3,6-Anhydro-α-L-galacto-β-D-galactan, Agarose ME
CAS Number:
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.25

biological source

algae (marine)

Quality Level

grade

for molecular biology

type

Type II

form

powder

impurities

≤10% moisture content

EEO

0.16-0.19

transition temp

congealing temperature 34.5-37.5 °C (1.5% gel)

gel strength

≥1000 g/cm2 (1.0% gel)

InChI

1S/C24H38O19/c25-1-5-9(27)11(29)12(30)22(38-5)41-17-8-4-36-20(17)15(33)24(40-8)43-18-10(28)6(2-26)39-23(14(18)32)42-16-7-3-35-19(16)13(31)21(34)37-7/h5-34H,1-4H2/t5-,6-,7+,8+,9+,10+,11+,12-,13+,14-,15+,16-,17-,18+,19+,20+,21-,22+,23+,24+/m1/s1

InChI key

MJQHZNBUODTQTK-WKGBVCLCSA-N

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A0169A2576A4718
Agarose Medium EEO, for molecular biology

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grade

for molecular biology

grade

-

grade

Molecular Biology

grade

for molecular biology

form

powder

form

powder

form

powder

form

powder

impurities

≤10% moisture content

impurities

≤7% water

impurities

≤7% water

impurities

-

EEO

0.16-0.19

EEO

0.09-0.13

EEO

≤0.12

EEO

≤0.05

transition temp

congealing temperature 34.5-37.5 °C (1.5% gel)

transition temp

gel point 36 °C ±1.5 °C (1.5% gel)

transition temp

gel point ≤20 °C (1.5% gel)

transition temp

gel point ≤35 °C (3% gel)

General description

Agarose is a polymer extracted from agar or agar-bearing marine algae. This purified linear galactan hydrocolloid comprises alternating co-polymers D-galactose and 3,6-anhydro-L-galactose units connected by α-(1→3) and β-(1→4) glycosidic bonds. Agarose is highly biocompatible, possesses variable mechanical and diffusion properties.

Application

Agarose has been used:
  • in gel electrophoresis
  • in the preparation of 2x agarose solution used along with 2x supplemented-MEM media as an overlay medium in plaque assay
  • in an immunodiffusion test for Paracoccidioidomycosis

Analysis Note

The following is a list of properties associated with our agaroses:
Sulfate content - used as an indicator of purity, since sulfate is the major ionic group present.
Gel strength - the force that must be applied to a gel to cause it to fracture.
Gel point - the temperature at which an aqueous agarose solution forms a gel as it cools. Agarose solutions exhibit hysteresis in the liquid-to-gel transition - that is, their gel point is not the same as their melting temperature.
Electroendosmosis (EEO) - a movement of liquid through the gel. Anionic groups in an agarose gel are affixed to the matrix and cannot move, but dissociable counter cations can migrate toward the cathode in the matrix, giving rise to EEO. Since electrophoretic movement of biopolymers is usually toward the anode, EEO can disrupt separations because of internal convection.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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