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A7174

Sigma-Aldrich

Agarose

High Gelling Temperature

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Synonym(s):
3,6-Anhydro-α-L-galacto-β-D-galactan
CAS Number:
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.25

Quality Level

type

Type VI-A

form

powder

impurities

≤10% water

ash

≤0.60%

EEO

≤0.14

transition temp

gel point 41 °C ±1.5 °C (1.5% gel)

gel strength

≥900 g/cm2 (1% gel)

anion traces

sulfate (SO42-): ≤0.20%

InChI

1S/C24H38O19/c25-1-5-9(27)11(29)12(30)22(38-5)41-17-8-4-36-20(17)15(33)24(40-8)43-18-10(28)6(2-26)39-23(14(18)32)42-16-7-3-35-19(16)13(31)21(34)37-7/h5-34H,1-4H2/t5-,6-,7+,8+,9+,10+,11+,12-,13+,14-,15+,16-,17-,18+,19+,20+,21-,22+,23+,24+/m1/s1

InChI key

MJQHZNBUODTQTK-WKGBVCLCSA-N

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This Item
A5030A6877A9918
Agarose High Gelling Temperature

A7174

Agarose

Agarose Ultra-low Gelling Temperature

A5030

Agarose

Agarose Medium EEO, for molecular biology

A6877

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Agarose Type II-A, Medium EEO

A9918

Agarose

form

powder

form

powder

form

powder

form

powder

gel strength

≥900 g/cm2 (1% gel)

gel strength

-

gel strength

≥1000 g/cm2 (1.0% gel)

gel strength

≥1000 g/cm2 (1% gel)

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

transition temp

gel point 41 °C ±1.5 °C (1.5% gel)

transition temp

gel point 8-17 °C

transition temp

congealing temperature 34.5-37.5 °C (1.5% gel)

transition temp

gel point 36 °C ±1.5 °C (1.5% gel)

anion traces

sulfate (SO42-): ≤0.20%

anion traces

-

anion traces

-

anion traces

sulfate (SO42-): ≤0.25%

Analysis Note

The following is a list of properties associated with our agaroses:
Sulfate content - used as an indicator of purity, since sulfate is the major ionic group present.
Gel strength - the force that must be applied to a gel to cause it to fracture.
Gel point - the temperature at which an aqueous agarose solution forms a gel as it cools. Agarose solutions exhibit hysteresis in the liquid-to-gel transition - that is, their gel point is not the same as their melting temperature.
Electroendosmosis (EEO) - a movement of liquid through the gel. Anionic groups in an agarose gel are affixed to the matrix and cannot move, but dissociable counter cations can migrate toward the cathode in the matrix, giving rise to EEO. Since electrophoretic movement of biopolymers is usually toward the anode, EEO can disrupt separations because of internal convection.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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