Merck
  • A8456
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A8456

Sigma-Aldrich

4-(2-Aminoethyl)benzenesulfonyl fluoride hydrochloride

≥97.0% (HPLC)

All Photos(2)
Synonym(s):
AEBSF
Empirical Formula (Hill Notation):
C8H10FNO2S · HCl
CAS Number:
30827-99-7
Molecular Weight:
239.69
Beilstein:
7604627
MDL number:
MFCD00132962
PubChem Substance ID:
24278240
NACRES:
NA.77

biological source

synthetic (organic)

Quality Level

300

Assay

≥97.0% (HPLC)

form

powder

mp

183-191  °C

solubility

H2O: 50 mg/mL (stable for up to six months if stored refrigerated at a pH of less than 7. If a pH of greater than 7 is required, pH adjustment should be made just prior to use.)

storage temp.

−20°C

SMILES string

FS(C1=CC=C(CCN)C=C1)(=O)=O.[H]Cl

InChI

1S/C8H10FNO2S.ClH/c9-13(11,12)8-3-1-7(2-4-8)5-6-10;/h1-4H,5-6,10H2;1H

InChI key

WRDABNWSWOHGMS-UHFFFAOYSA-N

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This Item
1.248391015005.08436
4-(2-Aminoethyl)benzenesulfonyl fluoride hydrochloride ≥97.0% (HPLC)

Sigma-Aldrich

A8456

4-(2-Aminoethyl)benzenesulfonyl fluoride hydrochloride

Pefabloc® SC (4-(2-Aminoethyl)-benzolsulfonylfluorid-hydrochloride) (AEBSF) for biochemistry

Millipore

1.24839

Pefabloc® SC (4-(2-Aminoethyl)-benzolsulfonylfluorid-hydrochloride)

AEBSF, Hydrochloride AEBSF, CAS 30827-99-7, is a specific irreversible inhibitor of chymotrypsin, kallikrein, plasmin, thrombin, trypsin, and related thrombolytic enzymes. A nontoxic alternative to PMSF and DFP.

Sigma-Aldrich

101500

AEBSF, Hydrochloride

InSolution AEBSF, Hydrochloride

Sigma-Aldrich

5.08436

InSolution AEBSF, Hydrochloride

biological source

synthetic (organic)

biological source

-

biological source

-

biological source

-

assay

≥97.0% (HPLC)

assay

≥98% (HPLC)

assay

>97% (HPLC)

assay

≥97% (HPLC)

form

powder

form

solid

form

solid

form

liquid

mp

183-191  °C

mp

175-177 °C

mp

-

mp

-

solubility

H2O: 50 mg/mL (stable for up to six months if stored refrigerated at a pH of less than 7. If a pH of greater than 7 is required, pH adjustment should be made just prior to use.)

solubility

10 g/L

solubility

water: 25 mg/mL

solubility

-

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−70°C

Application

4-(2-Aminoethyl)benzenesulfonyl fluoride hydrochloride (AEBSF) has been used:
  • as a protease inhibitor in plasma samples to prevent acylated ghrelin (AG) degradation
  • as a snake venom serine proteinase (SVSP) inhibitor in SVSP inhibition or AEBSF assay to test the involvement of SVSPs on the fibrinogen-clotting activity
  • as a component in NP-40 lysis buffer to resuspend cell pellets for preparing cell lysates

Recommended concentrations for use are 0.1-1 mM.

Biochem/physiol Actions

AEBSF can inhibit acetylcholinesterase (AChE).
4-(2-Aminoethyl)benzenesulfonyl fluoride hydrochloride (AEBSF) is an irreversible serine protease inhibitor. Inhibition constants are similar to those of phenylmethanesulfonyl fluoride (PMSF) and diisopropylfluorophosphate (DFP). AEBSF has been shown to inhibit trypsin, chymotrypsin, plasmin kallikrein, and thrombin. As an alternative to PMSF and DFP, AEBSF offers lower toxicity, improved solubility in water, and improved stability in aqueous solutions. AEBSF can be used in cell culture in concentrations of up to 0.25 mM.

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H315 - H319

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

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Certificate of Origin

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