A9793
Agarose
High EEO
Recommended Products
Quality Level
type
Type III-A
form
powder
impurities
≤7% water (loss on drying)
ash
≤1.1%
turbidity
≤4 NTU (1.5% gel)
EEO
0.23-0.26
transition temp
gel point 36 °C ±1.5 °C (1.5% gel)
gel strength
≥750 g/cm2 (1% gel)
anion traces
sulfate (SO42-): ≤0.25%
InChI
1S/C24H38O19/c25-1-5-9(27)11(29)12(30)22(38-5)41-17-8-4-36-20(17)15(33)24(40-8)43-18-10(28)6(2-26)39-23(14(18)32)42-16-7-3-35-19(16)13(31)21(34)37-7/h5-34H,1-4H2/t5-,6-,7+,8+,9+,10+,11+,12-,13+,14-,15+,16-,17-,18+,19+,20+,21-,22+,23+,24+/m1/s1
InChI key
MJQHZNBUODTQTK-WKGBVCLCSA-N
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This Item | A5304 | A0576 | A3643 |
|---|---|---|---|
| form powder | form powder | form powder | form powder |
| impurities ≤7% water (loss on drying) | impurities ≤11% moisture content | impurities ≤7% water | impurities - |
| EEO 0.23-0.26 | EEO ≤0.40 | EEO ≤0.12 | EEO ≥0.3 |
| transition temp gel point 36 °C ±1.5 °C (1.5% gel) | transition temp gel point 36 °C ±1.5 °C (1.5% gel) | transition temp gel point 36 °C ±1.5 °C (1.5% gel) | transition temp - |
| gel strength ≥750 g/cm2 (1% gel) | gel strength ≥700 g/cm2 (1% gel) | gel strength ≥1800 g/cm2 (1% gel), ≥3200 g/cm2 (1.5% gel) | gel strength - |
| anion traces sulfate (SO42-): ≤0.25% | anion traces sulfate (SO42-): ≤0.2% | anion traces sulfate (SO42-): ≤0.12% | anion traces - |
Analysis Note
Sulfate content - used as an indicator of purity, since sulfate is the major ionic group present.
Gel strength - the force that must be applied to a gel to cause it to fracture.
Gel point - the temperature at which an aqueous agarose solution forms a gel as it cools. Agarose solutions exhibit hysteresis in the liquid-to-gel transition - that is, their gel point is not the same as their melting temperature.
Electroendosmosis (EEO) - a movement of liquid through the gel. Anionic groups in an agarose gel are affixed to the matrix and cannot move, but dissociable counter cations can migrate toward the cathode in the matrix, giving rise to EEO. Since electrophoretic movement of biopolymers is usually toward the anode, EEO can disrupt separations because of internal convection.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
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