B4501

Biotin

≥99% (HPLC), lyophilized powder

• Synonym(s): D-Biotin, Bios II, Coenzyme R, Vitamin B7, Vitamin H
• Empirical Formula (Hill Notation): C₁₀H₁₆N₂O₃S
• CAS Number: 58-85-5
• Molecular Weight: 244.31
• Beilstein: 86838
• EC Number: 200-399-3
• MDL number: MFCD00005541
• eCl@ss: 34058011
• PubChem Substance ID: 24891778
• NACRES: NA.21

Properties

• biological source: synthetic (organic)
• Quality Level: 300
• Assay: ≥99% (HPLC)
• form: lyophilized powder
• technique(s): HPLC: suitable
• color: white to off-white
• mp: 231-233 °C (lit.)
• solubility: ammonium hydroxide: 50 mg/mL (2 M)
• storage temp.: 2-8°C
• SMILES string: [H][C@]12CS[C@@H](CCCCC(O)=O)[C@@]1([H])NC(=O)N2
• InChI: 1S/C10H16N2O3S/c13-8(14)4-2-1-3-7-9-6(5-16-7)11-10(15)12-9/h6-7,9H,1-5H2,(H,13,14)(H2,11,12,15)/t6-,7-,9-/m0/s1
• InChI key: YBJHBAHKTGYVGT-ZKWXMUAHSA-N

Description

Application

Biotin may be used to elute proteins from avidin/streptavidin resins.

• It has been used for culturing of oligodendrocytes.
• It has been used as a vitamin supplement for the growth of Bacillus species.
• It has been used for blocking endogenous biotin during immunohistology procedures.

Biochem/physiol Actions

Biotin is a small molecule and its presence with biologically active macromolecules does not affect the biological function of the macromolecule. The biotin-(strept)avidin system are required for various applications. Biotin also works as a cofactor of carboxylating enzymes, such as pyruvate carboxylase. It catalyzes the formation of oxaloacetate from pyruvate and CO₂. Biotin conjugation with avidin or streptavidin helps in linking the target molecule (antibody, nucleotide, protein A etc.) with the labeling system (enzyme, fluorescent, chemiluminescent probes). This complex is used for many detection systems, such as immunoassays, DNA hybridization assays, immunohistochemistry and flow-cytometry. This approach is also applied for the purification and characterization of various molecules of interest.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves