Merck
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B8026

Sigma-Aldrich

Bromophenol Blue sodium salt

for molecular biology, suitable for electrophoresis

Synonym(s):
3′,3′′,5′,5′′-Tetrabromophenolsulfophthalein sodium salt
Empirical Formula (Hill Notation):
C19H9Br4NaO5S
CAS Number:
Molecular Weight:
691.94
Beilstein:
6995701
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.47

Quality Level

grade

for molecular biology

form

powder

technique(s)

electrophoresis: suitable

visual transition interval

3.0-4.6, yellow to blue

ε (extinction coefficient)

≥150000 at 597-603 nm in H2O
≥17000 at 250-256 nm in H2O
≥23000 at 306-312 nm in H2O

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Na+].Oc1c(Br)cc(cc1Br)C(=C2/C=C(Br)C(=O)C(Br)=C2)\c3ccccc3S([O-])(=O)=O

InChI

1S/C19H10Br4O5S.Na/c20-12-5-9(6-13(21)18(12)24)17(10-7-14(22)19(25)15(23)8-10)11-3-1-2-4-16(11)29(26,27)28;/h1-8,24H,(H,26,27,28);/q;+1/p-1

InChI key

TWKDJMRHSLWRFI-UHFFFAOYSA-M

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General description

Bromophenol blue (BroB), an arylmethane color marker dye has a molecular mass of 670 Da.

Application

Bromophenol Blue sodium salt has been used in the buffer solution to differentiate dead and living cells. It has also been used as a constituent in gel loading buffer.
A tracking dye for nucleic acid or protein electrophoresis in agarose or polyacrylamide gels.

Packaging

5, 25 g in glass bottle

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

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