C1359

Calcein AM solution

4 mM in DMSO, ≥90% (HPLC), solution

• Empirical Formula (Hill Notation): C₄₆H₄₆N₂O₂₃
• CAS Number: 148504-34-1
• Molecular Weight: 994.86
• EC Number: 200-664-3
• MDL number: MFCD05861516
• PubChem Substance ID: 24892405
• NACRES: NA.47

Properties

• Quality Level: 100
• assay: ≥90% (HPLC)
• form: solution
• concentration: 4 mM in DMSO
• λmax: 491-497 nm
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: −20°C
• SMILES string: CC(=O)OCOC(=O)CN(CC(=O)OCOC(C)=O)Cc1c(OC(C)=O)ccc2c1Oc3c(CN(CC(=O)OCOC(C)=O)CC(=O)OCOC(C)=O)c(OC(C)=O)ccc3C24OC(=O)c5ccccc45
• InChI: 1S/C46H46N2O23/c1-25(49)60-21-64-39(55)17-47(18-40(56)65-22-61-26(2)50)15-32-37(68-29(5)53)13-11-35-43(32)70-44-33(16-48(19-41(57)66-23-62-27(3)51)20-42(58)67-24-63-28(4)52)38(69-30(6)54)14-12-36(44)46(35)34-10-8-7-9-31(34)45(59)71-46/h7-14H,15-24H2,1-6H3
• InChI key: XKFSBWQWNMZWFA-UHFFFAOYSA-N

Description

Application

In cells, calcein AM solution has been used in calcein-AM dye transfer assay. It has been used for the staining of cells to study quantification of cell-migration. It has been used to study cytotoxic effects of drugs in cells.

Packaging

100 μL in glass bottle

Biochem/physiol Actions

Fluorescent cell permeable derivative of calcein.

Calcein AM is a membrane permeable nonfluorescent molecule. It is converted to fluorescent calcein by intracellular esterases. Calcein has limited membrane permeability. Calcein AM is also a substrate for p-glycoprotein (Pgp). Presence of Pgp results in efflux of calcein AM and thereby decreases cellular fluorescence. On the other hand, presence of Pgp inhibitor increases cellular entry of calcein AM and thereby increases cellular fluorescence. Calcein AM plays an important role in early drug discovery.

Physical form

Solution in 0.4% Calcein AM and 99.6% DMSO.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point(F): 185.0 °F - closed cup
• Flash Point(C): 85 °C - closed cup
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves