H3136

Hematoxylin

certified by the Biological Stain Commission

• Synonym(s): Natural Black 1
• Empirical Formula (Hill Notation): C₁₆H₁₄O₆ · xH₂O
• CAS Number: 517-28-2
• Molecular Weight: 302.28 (anhydrous basis)
• Colour Index Number: 75290
• Beilstein: 91400
• EC Number: 208-237-3
• MDL number: MFCD00078111
• PubChem Substance ID: 24895554
• NACRES: NA.47

Properties

• grade: certified by the Biological Stain Commission
• Quality Level: 200
• form: powder
• mp: 200 °C (dec.) (lit.)
• solubility: ethanol: 1 mg/mL
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• SMILES string: Oc1cc2C[C@@]3(O)COc4c(O)c(O)ccc4[C@H]3c2cc1O
• InChI: 1S/C16H14O6/c17-10-2-1-8-13-9-4-12(19)11(18)3-7(9)5-16(13,21)6-22-15(8)14(10)20/h1-4,13,17-21H,5-6H2/t13-,16+/m0/s1
• InChI key: WZUVPPKBWHMQCE-XJKSGUPXSA-N
• Gene Information: rat ... Adora1(29290) , Adora2a(25369)

Description

General description

Hematoxylin is a basophilic dye extracted from the tree Heamatoxylum campechianum. It specifically binds to acidic compounds and thus is useful for staining cell nuclei which contain acidic substances: DNA and RNA. Hematoxylin, when oxidized forms hematein, which has thick pale blue purple colour. Hematoxylin is often used along with eosin, in histological study under light microscope.

Application

Hematoxylin has been used:

• to visualize anatomical features and to detect nucleus locus coeruleus adjacent to the periaqueductal gray region in brainstem samples
• as a nuclear counterstain to visualize the nucleus, adipocytes were counterstained with a hematoxylin reagent
• to stain lung tissue for histological analysis

Suitability

Certified for use in Weigert′s iron hematoxylin, Mayer′s hemalum, Harris hemalum and in Heidenhain′s technique

Safety Information

• Pictograms: GHS07
• Signal Word: Warning
• Hazard Statements: H319
• Precautionary Statements: P264 - P280 - P305 + P351 + P338 - P337 + P313
• Hazard Classifications: Eye Irrit. 2
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves