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H3136

Sigma-Aldrich

Hematoxylin

certified by the Biological Stain Commission

Synonym(s):
Natural Black 1
Empirical Formula (Hill Notation):
C16H14O6 · xH2O
CAS Number:
Molecular Weight:
302.28 (anhydrous basis)
Colour Index Number:
75290
Beilstein:
91400
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.47

Quality Level

grade

certified by the Biological Stain Commission

form

powder

application(s)

hematology: suitable
histology: suitable

mp

200 °C (dec.) (lit.)

solubility

ethanol: 1 mg/mL

Featured Industry

Diagnostic Assay Manufacturing

storage temp.

room temp

SMILES string

Oc1cc2C[C@@]3(O)COc4c(O)c(O)ccc4[C@H]3c2cc1O

InChI

1S/C16H14O6/c17-10-2-1-8-13-9-4-12(19)11(18)3-7(9)5-16(13,21)6-22-15(8)14(10)20/h1-4,13,17-21H,5-6H2/t13-,16+/m0/s1

InChI key

WZUVPPKBWHMQCE-XJKSGUPXSA-N

Gene Information

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General description

Hematoxylin is a basophilic dye extracted from the tree Heamatoxylum campechianum. It specifically binds to acidic compounds and thus is useful for staining cell nuclei which contain acidic substances: DNA and RNA. Hematoxylin, when oxidized forms hematein, which has thick pale blue purple colour. Hematoxylin is often used along with eosin, in histological study under light microscope.

Application

Hematoxylin has been used:
  • to visualize anatomical features and to detect nucleus locus coeruleus adjacent to the periaqueductal gray region in brainstem samples
  • as a nuclear counterstain to visualize the nucleus, adipocytes were counterstained with a hematoxylin reagent
  • to stain lung tissue for histological analysis

Packaging

25, 100 g in glass bottle

Suitability

Certified for use in Weigert′s iron hematoxylin, Mayer′s hemalum, Harris hemalum and in Heidenhain′s technique

pictograms

Exclamation mark

signalword

Warning

hcodes

Hazard Classifications

Eye Irrit. 2

Storage Class Code

11 - Combustible Solids

WGK Germany

WGK 1

Flash Point F

Not applicable

Flash Point C

Not applicable

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Certificate of Analysis

Certificate of Origin

Expression of matrilin-2 in liver cirrhosis and hepatocellular carcinoma.
Szabo E
Pathology Oncology Research, 14, 15-15 (2008)
Lung Section Staining and Microscopy
Zhou X and Moore BB
Bio-protocol (2017)
S-nitrosylation of fatty acid synthase regulates its activity through dimerization.
Choi MS
Journal of Lipid Research, 57, 607-607 (2016)
Techniques in Aquatic Toxicology (2005)
Histologic validation of locus coeruleus MRI contrast in post-mortem tissue.
Keren NI
Neuroimage, 113, 235-235 (2015)

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