Merck
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H3149

Sigma-Aldrich

Heparin sodium salt from porcine intestinal mucosa

Grade I-A, ≥180 USP units/mg, powder, BioReagent, suitable for cell culture

All Photos(8)
CAS Number:
9041-08-1
MDL number:
MFCD00081689
NACRES:
NA.21

biological source

Porcine intestinal mucosa

Quality Level

200

type

Grade I-A

product line

BioReagent

form

powder

specific activity

≥180 USP units/mg

technique(s)

cell culture | mammalian: suitable

solubility

H2O: 50 mg/mL

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General description

Heparin is mainly responsible for the delay in the coagulation of blood. It enhances the antithrombin-mediated inactivation of proteases in the coagulation pathway.

Application

  • Heparin sodium salt from porcine intestinal mucosa has been used in sLRP (soluble circulating low density lipoprotein receptor-related protein-1) purification from human plasma.
  • It has been used as a supplement in M199 medium for culturing of human umbilical vein endothelial cells.
  • It has been used as an anticoagulant agent during isolation of blood.

Packaging

10000, 25000, 50000, 100000, 250000, 500000, 1000000 units in poly bottle

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

  1. How is Heparin sodium salt from porcine intestinal mucosa, Product H3149, solubilized?

    Heparin sodium salt from porcine intestinal mucosa is soluble in deionized water at a concentrationi of 50 mg/mL.

  2. How much of Heparin sodium salt from porcine intestinal mucosa, Product H3149, is used for cell culture?

    Solutions at 0.5 mg per mL were tested in cell culture. Start at this concentration, and titer from there to determine the optimal concentration for usage.

  3. How are solutions Heparin sodium salt from porcine intestinal mucosa, Product H3149, stored?

    Solutions can be stored at 2-8C for up to 2 years, if sterile filtered through a 0.22 micron filter. Benzyl alcohol (0.06-1%) can be added to solutions to prevent microbial growth.

  4. How many milligrams of Heparin sodium salt, Product H3149, are in the bottle?

    The potency, in units/mg, is lot-specific; the value can be found on the certificate of analysis. It is typically over 140 units per mg. The number of milligrams can be calculated by dividing the number of units by the potency in units per mg.

  5. What is the molecular weight of Heparin sodium salt?

    Heparin is a mixture of polyanion chains having molecular weights ranging from 6,000 to 30,000 Daltons, with most chains in the range of 17,000 to 19,000 Daltons.

  6. How much Heparin sodium salt, is used to prevent coagulation?

    We suggest using 20 to 50 units per mL of blood.

  7. What is the best way to sterilize Heparin sodium salt?

    Although solutions may be autoclaved at 121 °C for 5 to 10 minutes, we generally recommend sterile filtration. This is because prolonged autoclaving may have an effect on its activity.  

  8. What concentration of Heparin sodium salt, can I use to inhibit RNAse activity?

    Heparin has been used at concentrations ranging from 100 to 500 μg/mL. This has been reported in the J. Biol. Chem., 248, 2095 (1973).

  9. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  10. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  11. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  12. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  13. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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