If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf
H3149
Heparin sodium salt from porcine intestinal mucosa
Grade I-A, ≥180 USP units/mg, powder, BioReagent, suitable for cell culture
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SMILES string
[Na+].[S](=O)(=O)(NC1C(OC(C(C1O[S](=O)(=O)O)OC4OC(C(C(C4O[S](=O)(=O)O)O)O)C(=O)O)CO)OC2C(OC(C(C2O)O[S](=O)(=O)O)OC3C(OC(C(C3O)NC(=O)C)O)CO[S](=O)(=O)O)C(=O)O)O
InChI
1S/C26H42N2O37S5.Na/c1-4(30)27-7-9(31)13(6(56-23(7)39)3-55-67(43,44)45)58-26-19(65-70(52,53)54)12(34)16(20(62-26)22(37)38)60-24-8(28-66(40,41)42)15(63-68(46,47)48)14(5(2-29)57-24)59-25-18(64-69(49,50)51)11(33)10(32)17(61-25)21(35)36;/h5-20,23-26,28-29,31-
InChI key
JRTRSJGZMRQDHI-UHFFFAOYSA-N
biological source
Porcine intestinal mucosa
type
Grade I-A
product line
BioReagent
form
powder
specific activity
≥180 USP units/mg
technique(s)
analytical sample preparation: suitable, cell culture | mammalian: suitable
color
beige
solubility
H2O: 50 mg/mL
application(s)
cell analysis
diagnostic assay manufacturing
life science and biopharma
sample preparation
Quality Level
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This Item | |||
|---|---|---|---|
| biological source Porcine intestinal mucosa | biological source Porcine intestinal mucosa | biological source Porcine intestinal mucosa | biological source Porcine intestinal mucosa |
| technique(s) analytical sample preparation: suitable, cell culture | mammalian: suitable | technique(s) - | technique(s) - | technique(s) - |
| solubility H2O: 50 mg/mL | solubility H2O: 50 mg/mL, clear, colorless to faintly yellow | solubility H2O: 50 mg/mL, clear, colorless to faintly yellow | solubility water: soluble 50 g/L, acetone: insoluble, alcohol: insoluble, benzene: insoluble, chloroform: insoluble, diethyl ether: insoluble |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| form powder | form powder | form powder | form powder |
| product line BioReagent | product line - | product line - | product line - |
General description
Application
- Heparin sodium salt from porcine intestinal mucosa has been used in sLRP (soluble circulating low density lipoprotein receptor-related protein-1) purification from human plasma.[2]
- It has been used as a supplement in M199 medium for culturing of human umbilical vein endothelial cells.[3]
- It has been used as an anticoagulant agent during isolation of blood.[4]
Features and Benefits
- High-purity compound suitable for a wide variety of research applications
Other Notes
comparable product
Storage Class
11 - Combustible Solids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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How can I determine the shelf life / expiration / retest date of this product?
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Please, can I have the monomer structure of H3149 heparin?
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No structure is reported for this product. Therefore, this information is not available.
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What is the amount in one vial? For example, 100KU vial contains 500 mg?
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The minimum activity of this product is 180 units per milligram. At the minimum activity, a 100KU vial will contain 555.6 mg. Typically, lots are released with an activity of more that 200 units per milligram. The lot specific activity is reported in the Certificate of Analysis. See the link below to review a sample or lot specific Certificate:
https://www.sigmaaldrich.com/product/sial/h3149#product-documentationHelpful?
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Can I dissolve the heparin in filtered (0.22 um) nuclease free water at a concentration of 50mg/ml? Does it correspond to 5000 IU/ml? Then, I can use 15-30 IU/ ml of blood to avoid its coagulation, is it correct?
1 answer-
As mentioned on the Product Detail Page, this product is soluble at 50 mg/mL in water. The actual activity is reported on the lot-specific Certificate of Analysis. This value should be used in determining the activity of the prepared solution. The activity specification for this product is >/=180 USP units/mg. For example, assuming an activity value of 200 units/mg, a prepared solution of 25 mg/ml will have an activity of 5000 units/ml.
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What is the storage condition of H3149 powder?
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This product should be stored in room temperature in powdered format.
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How is shipping temperature determined? And how is it related to the product storage temperature?
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Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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H3149-10KU heparin sodium salt is steril??
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The product is not sterile. Prepared solutions may be filter sterilized.
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please, could you explain me if this H3149 is one of 'high molecolar weight heparin'? thanks
1 answer-
This product is a cell culture tested porcine heparin with a molecular weight range of 15-19 kDa. This material is considered a "high molecular weight heparin." According to the literaure, low molecular weight heparins are those with a molecular wieght of less that 8000 Da. See the link below to review a publication that may be helpful:
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10141002/Helpful?
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Can this material be diluted to 10,000 U/mL and stored at 2C to 8C? Do you have stability data at this temperature for lower concentrations?
1 answer-
Yes, this product remains biologically active in dilute solutions. Stock solutions prepared at 50 mg/mL in water are stable for up to 2 years at 2 - 8°C, when filter sterilized. According to literature, heparin diluted in physiological saline at a concentration of 10 to 100 units/mL is stable for at least 35 days when stored at 2 - 8°C. Please see the link below to review the datasheet for an identical product, H3393, which may be applied to this item
H3393 Datasheet
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/112/608/h3393pis.pdfHelpful?
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Can you recommend a suitable heparin product for mouse heart perfusion?
1 answer-
Our available heparin products have not undergone specific testing for mouse heart perfusion. However, you may find H3149 of interest as it has been cited in the following publication for this intended application: "Isolation and Primary Culture of Mouse Aortic Endothelial Cells" published in J Vis Exp. 2016; (118): 52965 (PMID: 28060318).
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