Michael Pistorino et al.
Biotechnology progress, 25(5), 1364-1371 (2009-07-28)
The recent use of heterologous hosts to produce natural products has shown significant potential, although limitations still exist regarding optimal production titers. In this study, we utilize micro-scale cultures and well-defined screening methods to identify key medium components that influence...
Robert H White
Journal of bacteriology, 192(20), 5437-5440 (2010-08-17)
N-Ethylglutamate (NEG) was detected in Escherichia coli BL21 cells grown on LB broth, and it was found to occur at a concentration of ∼4 mM in these cells under these conditions. The same cells grown on M9 glucose medium contained...
Jigita Padhiar et al.
Pakistan journal of biological sciences : PJBS, 14(22), 1011-1018 (2012-04-21)
The present study was aimed at optimization, production and partial purification of lipases from Pseudomonas aeruginosa, Candida albicans and Aspergillus flavus. Various nutritional and physical parameters affecting lipase production such as carbon and nitrogen supplements, pH, temperature, agitation speed and...
Filomena Silva et al.
Journal of microbiology and biotechnology, 19(11), 1408-1414 (2009-12-10)
The obtention of high yields of purified plasmid DNA is viewed as an essential issue to be considered towards efficient production of DNA vaccines and therapeutic plasmids. In this work, Escherichia coli DH5alpha bearing the pVAX1-LacZ plasmid was grown in...
[Factors responsible for transition of the carnivorous fungus Duddingtonia flagrans from saprophytic to zootrophic type of nutrition ].
G G Anan'ko et al.
Mikrobiologiia, 80(2), 200-206 (2011-07-22)