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PHR1428

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HEPES

Pharmaceutical Secondary Standard; Certified Reference Material

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Synonym(s):
HEPES, 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)
Empirical Formula (Hill Notation):
C8H18N2O4S
CAS Number:
Molecular Weight:
238.30
Beilstein:
883043
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.24

grade

certified reference material
pharmaceutical secondary standard

Quality Level

API family

hepes

CofA

current certificate can be downloaded

technique(s)

HPLC: suitable
gas chromatography (GC): suitable

pH

5.0-6.5 (25 °C, 238 g/L)

useful pH range

6.8-8.2

pKa (25 °C)

7.5

application(s)

pharmaceutical (small molecule)

format

neat

storage temp.

2-30°C

SMILES string

OCCN1CCN(CC1)CCS(O)(=O)=O

InChI

1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)

InChI key

JKMHFZQWWAIEOD-UHFFFAOYSA-N

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This Item
H4034H7523H23830
HEPES Pharmaceutical Secondary Standard; Certified Reference Material

PHR1428

HEPES

-
HEPES BioPerformance Certified, ≥99.5% (titration), suitable for cell culture

H4034

HEPES

Premium Grade
HEPES BioXtra, pH 5.0-6.5 (1 M in H2O), ≥99.5% (titration)

H7523

HEPES

Premium Grade
HEPES reagent grade, ≥99% (titration), crystalline

H23830

HEPES

Essential Grade
application(s)

pharmaceutical (small molecule)

application(s)

clinical research
diagnostic assay manufacturing
general analytical
life science and biopharma

application(s)

diagnostic assay manufacturing

application(s)

-

format

neat

format

-

format

-

format

-

Quality Level

300

Quality Level

400

Quality Level

400

Quality Level

200

grade

certified reference material, pharmaceutical secondary standard

grade

BioPerformance Certified

grade

-

grade

reagent grade

storage temp.

2-30°C

storage temp.

-

storage temp.

-

storage temp.

-

General description

HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic and is effective as a buffer at pH 6.8 to 8.2.


Pharmaceutical secondary standards for application in quality control, provide pharma laboratories and manufacturers with a convenient and cost-effective alternative to the preparation of in-house working standards.

Application

These Secondary Standards are qualified as Certified Reference Materials. These are suitable for use in several analytical applications including but not limited to pharma release testing, pharma method development for qualitative and quantitative analyses, food and beverage quality control testing, and other calibration requirements.
  • HEPES has been used in a wide variety of applications, including tissue culture.
  • It is used to buffer cell culture media in air.
  • Finds its usage in invitro experiments on Mg.
  • Recognized as one of Dr.Good′s recommended buffers, used in some cell culture media as a buffering agent and also in various biochemical reactions.
  • Recently, in the production of radiopharmaceuticals, it is the buffer of choice for scientific labeling.

HEPES may also be used as:
  • Component of homogenization buffer to homogenize adipose tissue for liquid chromatography/mass spectrometry and in HN buffer for dissolving viral stock titer after dot blot hybridization.
  • Pharmaceutical reference standard for the determination of the analyte in bulk drug and pharmaceutical formulations by high performance liquid chromatography.

Analysis Note

These secondary standards offer multi-traceability to the USP, EP and BP primary standards, where they are available.

Other Notes

This Certified Reference Material (CRM) is produced and certified in accordance with ISO 17034 and ISO/IEC 17025. All information regarding the use of this CRM can be found on the certificate of analysis.

Footnote

To see an example of a Certificate of Analysis for this material enter LRAC3253 in the slot below. This is an example certificate only and may not be the lot that you receive.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

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Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

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HEPES BioUltra, for molecular biology, ≥99.5% (T)

Sigma-Aldrich

54457

HEPES

HEPES BioXtra, pH 5.0-6.5 (1 M in H2O), ≥99.5% (titration)

Sigma-Aldrich

H7523

HEPES

HEPES BioXtra, suitable for mouse embryo cell culture, ≥99.5% (titration)

Sigma-Aldrich

H6147

HEPES

HEPES hemisodium salt ≥99% (titration)

Sigma-Aldrich

H7637

HEPES hemisodium salt

HEPES ULTROL® Grade, ≥99% dry basis (titration), 1M zwitterionic N-substituted aminosulfonic acid buffer

Millipore

375368

HEPES

Determination of HEPES in 68 Ga-labeled peptide solutions
Sasson R, et al.
J. Radioanal. Nucl. Chem., 283(3), 753- 756 (2010)
FGF21 Prevents Angiotensin II-Induced Hypertension and Vascular Dysfunction by Activation of ACE2/Angiotensin-(1?7) Axis in Mice.
Pan X, et al.
Cell Metabolism (2018)
Simple and reliable chromatographic technique for the determination of HEPES in Ga-68-labeled pharmaceuticals
Maus S, et al.
Journal of Nuclear Medicine, 56(3), 1168- 1168 (2015)
Development and evaluation of a rapid analysis for HEPES determination in 68 Ga-radiotracers
Pfaff S, et al.
EJNMMI Research, 8(1), 95-95 (2018)
Hydrogen ion buffers for biological research.
Good NE, et al.
Biochemistry, 5(2), 467-477 (1966)

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