Merck
All Photos(3)

S2255

Sigma-Aldrich

Safranin O

Dye content ≥85 %

Synonym(s):
Basic Red 2, Gossypimine, Safranin Y or A, Safranin T, Cotton Red
Empirical Formula (Hill Notation):
C20H19ClN4
CAS Number:
Molecular Weight:
350.84
Colour Index Number:
50240
Beilstein:
3924099
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.47

form

powder

Quality Level

composition

Dye content, ≥85%

solubility

water: 1 mg/mL, clear, dark red to very dark red and red purple

εmax

1250-1650 at 530-534 nm in 50% ethanol

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Cl-].Cc1cc2nc3cc(C)c(N)cc3[n+](-c4ccccc4)c2cc1N

InChI

1S/C20H18N4.ClH/c1-12-8-17-19(10-15(12)21)24(14-6-4-3-5-7-14)20-11-16(22)13(2)9-18(20)23-17;/h3-11H,1-2H3,(H3,21,22);1H

InChI key

OARRHUQTFTUEOS-UHFFFAOYSA-N

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General description

Safranin O is a cationic dye, which displays less significant metachromasia. It is used to detect any variations in articular disease. Safranin O binds specifically to polyanions. It detects the amount of proteoglycan present in cartilages. Safranin O is used as a marker for glycosaminoglycan depletion in osteoarthritis. Safranin is used as a counter stain in Gram staining.

Application

Safranin O has been used in the histological analysis of femurs. It has also been used to detect the presence of glycosaminoglycans in the inner body of meniscus structures.

Packaging

25, 100 g in glass bottle

Pictograms

Corrosion

Signal Word

Danger

Hazard Statements

Precautionary Statements

Hazard Classifications

Eye Dam. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

Paul A. Banaszkiewicz
Classic Papers in Orthopaedics (2014)
Delivery of PDGF-B and BMP-7 by mesoporous bioglass/silk fibrin scaffolds for the repair of osteoporotic defects
Yufeng Zhang
Biomaterials (2012)
A new modification and application of the Gram stain
Hucker GJ
Journal of Bacteriology, 6(4), 395-395 (1921)
Classic Papers in Orthopaedics (2014)
Meniscus Structure in Human, Sheep, and Rabbit for Animal Models of
Meniscus Repair
Anik C
Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society (2009)

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