T3754
reagent grade
≥98% (HPLC)
powder
white to off-white
>300 °C (dec.) (lit.)
1 M HCl: soluble 50 mg/mL
detection
N[C@@H](Cc1ccc(O)cc1)C(O)=O
1S/C9H11NO3/c10-8(9(12)13)5-6-1-3-7(11)4-2-6/h1-4,8,11H,5,10H2,(H,12,13)/t8-/m0/s1
OUYCCCASQSFEME-QMMMGPOBSA-N
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11 - Combustible Solids
WGK 1
Not applicable
Not applicable
dust mask type N95 (US), Eyeshields, Gloves
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Proteinase K (EC 3.4.21.64) activity can be measured spectrophotometrically using hemoglobin as the substrate. Proteinase K hydrolyzes hemoglobin denatured with urea, and liberates Folin-postive amino acids and peptides. One unit will hydrolyze hemoglobin to produce color equivalent to 1.0 μmol of tyrosine per minute at pH 7.5 at 37 °C (color by Folin & Ciocalteu's Phenol Reagent).
Measure and compare the activity of proteases with this non-specific protease activity assay using casein. Use this assay as a standardized procedure to determine the activity of proteases for quality control.
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