34885

Sigma-Aldrich

Methanol

suitable for HPLC, gradient grade, ≥99.9%

Synonym(s):
Methyl alcohol
Linear Formula:
CH3OH
CAS Number:
Molecular Weight:
32.04
Beilstein/REAXYS Number:
1098229
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.05

grade

gradient grade

Quality Level

vapor density

1.11 (vs air)

vapor pressure

410 mmHg ( 50 °C)
97.68 mmHg ( 20 °C)

assay

≥99.9%

form

liquid

autoignition temp.

725 °F

expl. lim.

36 %

application(s)

HPLC: suitable
immunofluorescence: suitable

impurities

KMnO4 red. matter (as O), in accordance
≤0.0005% free alkali (as NH3)
≤0.0005% non-volatile matter
≤0.001% acetaldehyde
≤0.001% acetone (GC)
≤0.001% formaldehyde
≤0.02% water (Karl Fischer)
≤1 ppb fluorescence (quinine) at 254 nm
≤1 ppb fluorescence (quinine) at 365nm

evapn. residue

<0.0003%

color

APHA: ≤10

refractive index

n20/D 1.329 (lit.)

bp

64.7 °C (lit.)

mp

−98 °C (lit.)

density

0.791 g/mL at 25 °C (lit.)

HPLC-gradient

≤2 mAU at 230 nm
≤5 mAU at 254 nm

absorption

in accordance

UV absorption

λ: 210 nm Amax: 0.50
λ: 220 nm Amax: 0.30
λ: 230 nm Amax: 0.15
λ: 235 nm Amax: 0.10
λ: 240 nm Amax: 0.05
λ: 260 nm Amax: 0.01
λ: 400 nm Amax: 0.01

Featured Industry

Food and Beverages

SMILES string

CO

InChI

1S/CH4O/c1-2/h2H,1H3

InChI key

OKKJLVBELUTLKV-UHFFFAOYSA-N

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General description

Thermochemical conversion of methanol to C2-C10 hydrocarbons in the presence of shape-selective zeolites has been reported. Its oxidation on Ru-Pt catalyst system by ruthenium ad-atoms has been proposed.

Application

Methanol (MeOH) has been used as an eluent in the HPLC estimation of malondialdehyde in plasma, which is an indicator of oxidative stress.
It may be used in the following studies:
  • Colony forming unit-fibroblast assay of bone marrow mononuclear cells.
  • As solvent for the preparation of extracts of hyphae of Aspergillus for the estimation of gliotoxin by reversed phase-HPLC.
  • Immunofluorescence studies.
  • To compose eluent for the ion-pair reverse-phase HPLC isolation of nucleotides and their decomposition products.

Packaging

1 pkg in Pure-Pac™ 2
1, 6×1, 2, 4, 4×4 L in glass bottle
100 mL in glass bottle

Preparation Note

Product filtered through a 0.2 μm filter

Other Notes

Pure-Pac® II containers require the Micromatic MacroValve coupler for dispensing solvents, Z560723.
Important notice
  • The article number 34885-4X2.5L-R will be discontinued. Please order the single bottle 34885-2.5L-R which is physically identical with the same exact specifications.

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Legal Information

Pure-Pac is a registered trademark of Sigma-Aldrich Co. LLC

signalword

Danger

hazcat

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Flam. Liq. 2 - STOT SE 1

storage_class_code

3 - Flammable liquids

WGK Germany

WGK 2

Flash Point F

49.5 °F - closed cup

Flash Point C

9.7 °C - closed cup

Certificate of Analysis

Certificate of Origin

R A Cunha et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 18(6), 1987-1995 (1998-05-15)
ATP analogs substituted in the gamma-phosphorus (ATPgammaS, beta, gamma-imido-ATP, and beta,gamma-methylene-ATP) were used to probe the involvement of P2 receptors in the modulation of synaptic transmission in the hippocampus, because their extracellular catabolism was virtually not detected in CA1 slices....
Ufuk Cobanoglu et al.
Asian Pacific journal of cancer prevention : APJCP, 12(6), 1399-1403 (2011-12-01)
Early diagnosis and prevention is very important for lung cancer patients. Previous studies have emphasized that the level of coenzyme Q10 (CoQ10), present primarily in mitochondria, decreases with age and is low in patients with chronic diseases. Our goal was...
Emer P Reeves et al.
Mycopathologia, 158(1), 73-79 (2004-10-19)
Aspergillus fumigatus is a pathogenic fungus capable of causing both allergic lung disease and invasive aspergillosis, a serious, life-threatening condition in neutropenic patients. Aspergilli express an array of mycotoxins and enzymes which may facilitate fungal colonisation of host tissue. In...
Robert E Schwartz et al.
The Journal of clinical investigation, 109(10), 1291-1302 (2002-05-22)
We have derived from normal human, mouse, and rat postnatal bone marrow primitive, multipotent adult progenitor cells (MAPCs) that can differentiate into most mesodermal cells and neuroectodermal cells in vitro and into all embryonic lineages in vivo. Here, we show...
Electrocatalysis by ad-atoms: Part II. Enhancement of the oxidation of methanol on platinum by ruthenium ad-atoms.
Watanabe MA and Motoo S.
J. Electroanal. Chem. Interfac. Electrochem., 60(3), 267-273 (1975)

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