Merck
All Photos(4)

60220

Sigma-Aldrich

Potassium phosphate monobasic

buffer substance, anhydrous, puriss. p.a., ACS reagent, reag. ISO, reag. Ph. Eur., 99.5-100.5%

Synonym(s):
prim.-Potassium phosphate, Monopotassium phosphate, Potassium dihydrogen phosphate
Linear Formula:
KH2PO4
CAS Number:
Molecular Weight:
136.09
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.21

Quality Level

grade

ACS reagent
anhydrous
puriss. p.a.

Agency

reag. ISO
reag. Ph. Eur.

assay

99.5-100.5%

form

crystalline

impurities

≤0.001% total nitrogen (N)

loss

≤0.2% loss on drying, 105 °C

pH

4.2-4.5 (20 °C, 5%)

pKa (25 °C)

(1) 2.15, (2) 6.82, (3) 12.38 (phosphoric acid)

mp

252.6 °C (lit.)

solubility

water: 0.5 g/10 mL, clear, colorless to light yellow

density

2.338 g/mL at 25 °C (lit.)

anion traces

chloride (Cl-): ≤5 mg/kg
sulfate (SO42-): ≤30 mg/kg

cation traces

As: ≤0.5 mg/kg
Ca: ≤10 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤15 mg/kg
Cu: ≤5 mg/kg
Fe: ≤5 mg/kg
Mg: ≤5 mg/kg
Mn: ≤5 mg/kg
Na: ≤50 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Zn: ≤5 mg/kg
heavy metals: ≤0.001% (by ICP)

SMILES string

[K+].OP(O)([O-])=O

InChI

1S/K.H3O4P/c;1-5(2,3)4/h;(H3,1,2,3,4)/q+1;/p-1

InChI key

GNSKLFRGEWLPPA-UHFFFAOYSA-M

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General description

Potassium phosphate is widely used in buffers to prepare biological samples and examine biological interactions.

Application

Potassium phosphate monobasic has been used:
  • to prepare the working luminol solution to study the prooxidant activity in the chemiluminescent system luminol–H2O2
  • in phosphate buffer saline (PBS) to culture embryonic stem cells (ESCs) and generate embryoid bodies
  • in PBS to prepare polydimethylsiloxane (PDMS) substrate

Packaging

1, 2.5 kg in poly bottle
500 g in poly bottle

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

More documents

Quotes and Ordering

Prooxidant potential of \_2 nanoparticles towards hydrogen peroxide
Sozarukova MM, et al.
Nanosystems: Physics, Chemistry, Mathematics, 12(3), 283-290 (2021)
Jules D P Valentin et al.
Journal of colloid and interface science, 552, 247-257 (2019-05-28)
Many materials used in the medical settings such as catheters and contact lenses as well as most biological tissues are not purely elastic, but rather viscoelastic. While substrate elasticity has been investigated for its influence on bacterial adhesion, the impact
Sonal Saxena et al.
MethodsX, 7, 101073-101073 (2020-10-22)
Investigation on the effects of disease-associated mutations on neurodevelopment is an essential approach to understand the molecular basis of neurological disorders and can be achieved by generating suitable animal models. However, some of the mutations preclude development of animal models
Casper Skovgaard et al.
Journal of applied physiology (Bethesda, Md. : 1985), 117(10), 1097-1109 (2014-09-06)
The purpose of this study was to examine whether speed endurance training (SET, repeated 30-s sprints) and heavy resistance training (HRT, 80-90% of 1 repetition maximum) performed in succession are compatible and lead to performance improvements in moderately trained endurance
Xinyan Wu et al.
Nature communications, 5, 4961-4961 (2014-09-24)
The PIK3CA gene is frequently mutated in human cancers. Here we carry out a SILAC-based quantitative phosphoproteomic analysis using isogenic knockin cell lines containing 'driver' oncogenic mutations of PIK3CA to dissect the signalling mechanisms responsible for oncogenic phenotypes induced by

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