03978

Methylene Blue solution

for microscopy, concentrate according to Ehrlich, concentrated, aqueous solution

• Synonym(s): Methylene blue stain, Ehrlich’s reagent III, Loeffler’s Methylene Blue
• Empirical Formula (Hill Notation): C₁₆H₁₈ClN₃S
• CAS Number: 61-73-4
• Molecular Weight: 319.85
• Beilstein: 3641570
• MDL number: MFCD00012111
• PubChem Substance ID: 329747853
• NACRES: NA.47

Properties

• grade: for microscopy
• Quality Level: 100
• form: liquid
• quality: concentrated, aqueous solution
• shelf life: ~3 yr
• concentration: 1.5% (1.5 g/ 100 mL)
• density: 1.0 g/mL at 20 °C
• εmax: 9.4 at 656-666 nm in water
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• SMILES string: [Cl-].CN(C)c1ccc2N=C3C=C\C(C=C3Sc2c1)=[N+](/C)C
• InChI: 1S/C16H18N3S.ClH/c1-18(2)11-5-7-13-15(9-11)20-16-10-12(19(3)4)6-8-14(16)17-13;/h5-10H,1-4H3;1H/q+1;/p-1
• InChI key: CXKWCBBOMKCUKX-UHFFFAOYSA-M

Description

General description

Methylene blue is used to stain animal cells to make their nuclei more observable. It is a component of staining media such as Wright′s stain and Jenner′s stain.

Methylene blue, also known as methylthioninium chloride, is a photoactive phenothiazine dye.[1] It is a small molecular weight dye,[2] which is effective against Gram positive and Gram negative bacteria.[3]

Application

Methylene Blue solution has been used to stain human amniotic fluid stem cells to determine cell viability.[4] It has also been used to stain sections to determine the presence of mycobacteria.[5][6]

Methylene blue is used to stain blood films/smears used in cytology and to stain RNA or DNA for viewing under the microscope or on hybridization membranes.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves