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Atto 425-Streptavidin; Applied to the development of fluorescence binding assays to detect volatile organic compounds in both the liquid and gas phase.

Atto 425-Streptavidin BioReagent, suitable for fluorescence, ≥95% (HPCE) | Sigma-Aldrich

BioReagent, suitable for fluorescence, &#8805;95% (HPCE)

Atto 425-Streptavidin

Nanoscale organization of nicotinic aceylcholine receptors by stimulated emission depletion microscopy.

Naked Dense Bodies Provoke Depression.

Post-fusion structural changes and their roles in exocytosis and endocytosis of dense-core vesicles.

Sub-Diffraction Nano Manipulation Using STED AFM.

Maturation of active zone assembly by Drosophila Bruchpilot.

Fluorescent markers emitting in the red are extremely valuable in biological microscopy since they minimize cellular autofluorescence and increase flexibility in multicolor experiments. Novel rhodamine dyes excitable with 630 nm laser light and emitting at around 660 nm have been developed. The new rhodamines are very photostable and have high fluorescence quantum yields of up to 80 %, long excited state lifetimes of 3.4 ns, and comparatively low intersystem-crossing rates. They perform very well both in conventional and in subdiffraction-resolution microscopy such as STED (stimulated emission depletion) and GSDIM (ground-state depletion with individual molecular return), as well as in single-molecule-based experiments such as fluorescence correlation spectroscopy (FCS). Syntheses of lipophilic and hydrophilic derivatives starting from the same chromophore-containing scaffold are described. Introduction of two sulfo groups provides high solubility in water and a considerable rise in fluorescence quantum yield. The attachment of amino or thiol reactive groups allows the dyes to be used as fluorescent markers in biology. Dyes deuterated at certain positions have narrow and symmetrical molecular mass distribution patterns, and are proposed as new tags in MS or LC-MS for identification and quantification of various substance classes (e.g., amines and thiols) in complex mixtures. High-resolution GSDIM images and live-cell STED-FCS experiments on labeled microtubules and lipids prove the versatility of the novel probes for modern fluorescence microscopy and nanoscopy.

Red-emitting rhodamine dyes for fluorescence microscopy and nanoscopy.

We describe a STED microscope optimized for colocalization experiments with up to three colors. Two fluorescence labels are separated by their fluorescence lifetime whereas a third channel is discriminated by the wavelength of fluorescence emission. Since it does not require a second STED beam, separating by lifetime is insensitive to drift and thus optimally suited for colocalization analyses. Furthermore, we propose a setup having a second STED beam for long duration multicolor recording.

Simultaneous multi-lifetime multi-color STED imaging for colocalization analyses.

SNARE Function Is Not Involved in Early Endosome Docking.

Munc18-1 Tuning of Vesicle Merger and Fusion Pore Properties.

Synaptic membrane proteins form stable microdomains in early endosomes.

STED Nanoscopy in Living Cells Using Fluorogen Activating Proteins.

Analysis of replication factories in human cells by super-resolution light microscopy.

Fluorescence lifetime imaging with pulsed diode laser enabled stimulated emission.

STED nanoscopy. A glimpse into the future.

Direct observation of the nanoscale dynamics of membrane lipids in a living cell. Nature.

Fluoreszierende Rhodamine und fluorogene Carbopyronine fur die STED-Mikroskopie lebender Zellen.

A novel nanoscopic tool by combining AFM with STED microscopy.

STED nanoscopy combined with optical tweezers reveals protein dynamics on densely covered DNA.

Self-Calibrated Line-Scan STED-FCS to Quantify Lipid Dynamics in Model and Cell Membranes.

Stimulated emission depletion-based raster image correlation spectroscopy reveals biomolecular dynamics in live cells.

Atto dyes are a series of fluorescent dyes that meet the critical needs of modern fluorescent technologies.

Atto Dyes for Superior Fluorescent Imaging

Atto 425-Streptavidin

BioReagent, suitable for fluorescence, ≥95% (HPCE)

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