122-20

Rat Tail Collagen Coating Solution (20 ML)

• NACRES: NA.75

Properties

• sterility: sterile; sterile-filtered
• Quality Level: 100
• form: liquid
• shelf life: 6 mo.
• packaging: pkg of 20 mL
• technique(s): cell culture | mammalian: suitable
• UniProt accession no.: P02454
• shipped in: wet ice
• storage temp.: 2-8°C
• Gene Information: rat ... Col1a1(29393)

Description

General description

Rat Tail Collagen Coating Solution (20 ML) enhances cell attachment and proliferation, or promotion of cell-specific morphology and function. It is commonly used for endothelial cells, hepatocytes, muscle cells, and a variety of other cell types.
This collagen solution is prepared in 0.02 M Acetic Acid at 50 ug/ml and sterile filtered.

Application

Coat tissue culture ware with Rat Tail Collagen Solution to culture surface ratio of 5 ug/cm². For example: 125 ug (2.5 ml) for a T-25 flask. 10.5 ug (210 ul) for each well of a 24-well plate.

Coat tissue culture ware at 37^oC for 2 hours. Carefully aspirate Collagen Coating Solution. Rinse the coated surface twice with PBS. Coated tissue culture ware may be used immediately or air-dried and stored at 4 ^oC for up to one week.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable