All Photos(1)

125-50

SAFC

Collagen Coating Solution (50 ML)

NACRES:
NA.75

Quality Level

form

liquid

shelf life

1 yr

shipped in

wet ice

storage temp.

2-8°C

General description

Collagen is a major fibrous, structural protein in animals. It is a key component of the extracellular matrix (ECM) and is predominantly present in the connective tissue, ligaments, tendons, and cornea. Collagens consist of three parallel left-handed polypeptide chains coiled to form a right-handed triple helix. They also have a globular domain.

Application

The coating concentration is 1 ml per 10 cm2 surface area of the cell culture ware: 2.5 ml for coating a T-25 flask or a 60 mm tissue culture dish. 7.5 ml for coating a T-75 flask or a 100 mm tissue culture dish.

Add Attachment Factor Solution to the flask and rock the flask gently to distribute the solution evenly to cover the whole culture surface. Coat the culture ware surface for 30 minutes at 37°C or 2 hours or overnight at room temperature. Remove Attachment Factor Solution by aspiration in a sterile hood. The coated flask can be used immediately or store at 4°C for up to 2 weeks.

Biochem/physiol Actions

Collagen is a basic unit for the growth and support of various cells and tissues in the body. Collagen may be involved in wound healing. Collagen might promote cell adhesion and proliferation when used as a coating on iron-manganese alloys (Fe-30%Mn) scaffolds in orthopedic applications.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Collagen: an overview
Patino MG, et al.
Implant Dentistry (2002)
Collagen Coating Effects on Fe--Mn Bioresorbable Alloys
Huang S, et al.
Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society (2020)
Henry Bautista-Amorocho et al.
BMC molecular and cell biology, 22(1), 1-1 (2021-01-08)
Culturing primary epithelial cells has a major advantage over tumor-derived or immortalized cell lines as long as their functional phenotype and genetic makeup are mainly maintained. The swine model has shown to be helpful and reliable when used as a...
Caroline H T Hall et al.
Gastroenterology, 159(3), 984-998 (2020-05-21)
Patients with inflammatory bowel diseases (IBDs) have intestinal barrier dysfunction. Creatine regulates energy distribution within cells and reduces the severity of colitis in mice. We studied the functions of the creatine transporter solute carrier family 6 member 8 (SLC6A8, also...
Wenqiang Quan et al.
Frontiers in cellular neuroscience, 14, 276-276 (2020-09-26)
Pericytes play a central role in regulating the structure and function of capillaries in the brain. However, molecular mechanisms that drive pericyte proliferation and differentiation are unclear. In our study, we immunostained NACHT, LRR and PYD domains-containing protein 3 (NLRP3)-deficient...

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