16951

Atto 495

BioReagent, suitable for fluorescence, ≥98.0% (HPCE)

• MDL number: MFCD07370099
• PubChem Substance ID: 329751418

Properties

• Quality Level: 100
• product line: BioReagent
• assay: ≥98.0% (HPCE)
• form: powder
• manufacturer/tradename: ATTO-TEC GmbH
• solubility: DMF: soluble; DMF: soluble; DMSO: soluble; H₂O: soluble; H₂O: soluble; acetone: soluble; acetone: soluble
• fluorescence: λ_ex 499 nm; λ_em 526 nm in 0.1 M phosphate pH 7.0
• suitability: suitable for fluorescence
• storage temp.: −20°C
• SMILES string: [O-]Cl(=O)(=O)=O.CN(C)c1ccc2C=C3C=C\C(C=C3N(CCCC(O)=O)c2c1)=[N+](/C)C
• InChI: 1S/C21H25N3O2.ClHO4/c1-22(2)17-9-7-15-12-16-8-10-18(23(3)4)14-20(16)24(19(15)13-17)11-5-6-21(25)26;2-1(3,4)5/h7-10,12-14H,5-6,11H2,1-4H3;(H,2,3,4,5)
• InChI key: KCYUESOHZRSRGP-UHFFFAOYSA-N

Description

General description

Atto 495 is a new label for proteins and oligo-nucleotides with high molecular absorption (80.000) and quantum yield (0.45) as well as sufficient Stoke′s shift between excitation and emission maximum. Atto 495 is characterized by a high photostability and thermostability. It is slightly hydrophilic, and solubility in polar solvents as DMF or DMSO is high.

Application

Atto 495 is a lipophilic fluorescent compound, which has served as the neutral analyte in the model, along with SDS as the charged micelle acting as a carrier to transport this solute in an electric field. Atto 495 was included as fluorescence EOF marker for chip electrophoresis. This reagent is zwitterionic at pH 8.3 and thus migrates as a neutral substance under the proper conditions.

Atto fluorescent labels are designed for high sensitivity applications, including single molecule detection. Atto labels have rigid structures that do not show any cis-trans-isomerization. Thus these labels display exceptional intensity with minimal spectral shift on conjugation.

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.