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16951

Sigma-Aldrich

Atto 495

BioReagent, suitable for fluorescence, ≥98.0% (HPCE)

MDL number:
PubChem Substance ID:

Quality Level

product line

BioReagent

assay

≥98.0% (HPCE)

form

powder

manufacturer/tradename

ATTO-TEC GmbH

solubility

DMF: soluble
DMF: soluble
DMSO: soluble
H2O: soluble
H2O: soluble
acetone: soluble
acetone: soluble

fluorescence

λex 499 nm; λem 526 nm in 0.1 M phosphate pH 7.0

suitability

suitable for fluorescence

storage temp.

−20°C

SMILES string

[O-]Cl(=O)(=O)=O.CN(C)c1ccc2C=C3C=C\C(C=C3N(CCCC(O)=O)c2c1)=[N+](/C)C

InChI

1S/C21H25N3O2.ClHO4/c1-22(2)17-9-7-15-12-16-8-10-18(23(3)4)14-20(16)24(19(15)13-17)11-5-6-21(25)26;2-1(3,4)5/h7-10,12-14H,5-6,11H2,1-4H3;(H,2,3,4,5)

InChI key

KCYUESOHZRSRGP-UHFFFAOYSA-N

General description

Atto 495 is a new label for proteins and oligo-nucleotides with high molecular absorption (80.000) and quantum yield (0.45) as well as sufficient Stoke′s shift between excitation and emission maximum. Atto 495 is characterized by a high photostability and thermostability. It is slightly hydrophilic, and solubility in polar solvents as DMF or DMSO is high.

Application

Atto 495 is a lipophilic fluorescent compound, which has served as the neutral analyte in the model, along with SDS as the charged micelle acting as a carrier to transport this solute in an electric field. Atto 495 was included as fluorescence EOF marker for chip electrophoresis. This reagent is zwitterionic at pH 8.3 and thus migrates as a neutral substance under the proper conditions.
Atto fluorescent labels are designed for high sensitivity applications, including single molecule detection. Atto labels have rigid structures that do not show any cis-trans-isomerization. Thus these labels display exceptional intensity with minimal spectral shift on conjugation.

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Certificate of Analysis

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Certificate of Origin

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