Merck
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18507

Sigma-Aldrich

Atto 565 maleimide

BioReagent, suitable for fluorescence

Empirical Formula (Hill Notation):
C37H37ClN4O10
Molecular Weight:
733.16
MDL number:
NACRES:
NA.32

Quality Level

product line

BioReagent

form

powder

manufacturer/tradename

ATTO-TEC GmbH

fluorescence

λex 563 nm; λem 592 nm in 0.1 M phosphate pH 7.0

suitability

corresponds for coupling to thiols
suitable for fluorescence

storage temp.

−20°C

Application

Atto 565 is a new label with high molecular absorption (120.000) and quantum yield (0.9) as well as sufficient stoke′s shift (excitation maximum 563 nm, emission maximum 592 nm). Due to an insignificant triplet formation rate it is well suited for single molecule detection applications. Maleimides are well suited for coupling to thiol groups. This is similar to iodacetamides, but maleimides do react more thiol selective. They do not show significant reaction with histidine or methionine. Hydrolysis of maleimides to a mixture of isomeric nonreactive maleamic acids can compete significantly with thiol modification, particularly above pH 8. Maleimides may be used for labeling of amines, which usually requires a higher pH than reaction of maleimides with thiols.

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

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Certificate of Origin

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