Merck
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47140

Sigma-Aldrich

4-Fluoro-7-nitrobenzofurazan

BioReagent, suitable for fluorescence, ≥98.0% (HPLC)

Synonym(s):
NBD-F
Empirical Formula (Hill Notation):
C6H2FN3O3
CAS Number:
Molecular Weight:
183.10
Beilstein:
1077571
MDL number:
PubChem Substance ID:
NACRES:
NA.32

product line

BioReagent

assay

≥98.0% (HPLC)

mp

52-54 °C (lit.)

fluorescence

λex 470 nm; λem 528 nm in ethanol
λex 470 nm; λem 550 nm (Amino acid adducts)

suitability

suitable for fluorescence

storage temp.

2-8°C

SMILES string

[O-][N+](=O)c1ccc(F)c2nonc12

InChI

1S/C6H2FN3O3/c7-3-1-2-4(10(11)12)6-5(3)8-13-9-6/h1-2H

InChI key

PGZIDERTDJHJFY-UHFFFAOYSA-N

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Application

4-Fluoro-7-nitrobenzofurazan (NBD-F) is used as a protein, amino acid and drug fluorescent labeling reagent in HPLC pre-column derivatization procedures.
Useful for fluorescent labeling of amino acids and amines in HPLC

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

235.4 °F - closed cup

Flash Point(C)

113 °C - closed cup

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

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Certificate of Origin

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More documents

Quotes and Ordering

Hongyi Zhang et al.
Analytical and bioanalytical chemistry, 386(5), 1387-1394 (2006-09-06)
An in-capillary derivatization of amino acids and peptides with 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F) was developed for their subsequent capillary electrophoretic analysis with laser-induced fluorescence detection (lambda (ex)=488 nm). The in-capillary derivatization was achieved in zone-passing mode by introducing successive plugs of sample
Hua-Ming Tseng et al.
Analytical and bioanalytical chemistry, 388(2), 433-439 (2007-03-21)
A rapid and sensitive capillary electrophoresis (CE) method has been developed for profiling organic metabolites containing amine functional groups in mammalian biofluids. Metabolites containing an amine group were derivatized with 4-fluoro-7-nitrobenzo-2,1,3-oxadiazol (NBD-F), separated by micellar electrokinetic chromatography (MEKC), and detected
Zsolt Wagner et al.
Electrophoresis, 32(20), 2816-2822 (2011-10-20)
Three different fluorescent tags have been compared for the quantitative analysis of aspartate and glutamate in brain microdialysate samples. Separation conditions have been optimized to achieve short analysis time using reversed polarity separation in coated capillary. Method validation has revealed
Hua-Ming Tseng et al.
Electrophoresis, 30(8), 1399-1405 (2009-03-26)
A sensitive CE with LIF method has been developed for quantitative analysis of small carbohydrates. In this work, 17 carbohydrates including mono-, di- and oligosaccharides were simultaneously derivatized with 4-fluoro-7-nitrobenzofurazane (NBD-F) via a two-step reaction involving reductive amination with ammonia
Chiaki Aoyama et al.
Analytical sciences : the international journal of the Japan Society for Analytical Chemistry, 25(1), 63-65 (2009-01-14)
A simple and sensitive determination method for selenomethionine (Se-Met) using an HPLC-fluorescence detection system coupled with an on-line electrochemical reactor has been developed. NBD-F (4-fluoro-7-nitro-2,1,3-benzoxadiazole) was used as the fluorescent derivatization reagent for Se-Met. NBD-Se-Met was separated from NBD-derivatives of

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