47608

Formaldehyde solution

for molecular biology, BioReagent, ≥36.0% in H₂O (T)

• Synonym(s): Formalin
• Linear Formula: HCHO
• CAS Number: 50-00-0
• Molecular Weight: 30.03
• Beilstein: 1209228
• MDL number: MFCD00003274
• PubChem Substance ID: 329757328
• NACRES: NA.52

Properties

• grade: for molecular biology
• Quality Level: 100
• vapor density: 1.03 (vs air)
• vapor pressure: 52 mmHg ( 37 °C)
• product line: BioReagent
• assay: 36.0-38.0% (aldehyde method)
• form: liquid
• autoignition temp.: 572 °F
• contains: ~10% methanol as stabilizer
• concentration: ≥36.0% in H₂O (T)
• technique(s): electrophoresis: suitable
• impurities: ≤0.03% free acid (as HCOOH)
• ign. residue: ≤0.05% (as SO₄)
• pH: 2.8-4.0
• density: 1.09 g/mL at 20 °C; 1.09 g/mL at 25 °C (lit.)
• anion traces: chloride (Cl^-): ≤10 mg/kg; sulfate (SO₄^2-): ≤20 mg/kg
• cation traces: Ca: ≤5 mg/kg; Cd: ≤1 mg/kg; Co: ≤1 mg/kg; Cr: ≤1 mg/kg; Cu: ≤1 mg/kg; Fe: ≤1 mg/kg; K: ≤20 mg/kg; Mg: ≤1 mg/kg; Mn: ≤1 mg/kg; Na: ≤100 mg/kg; Ni: ≤1 mg/kg; Pb: ≤1 mg/kg; Zn: ≤1 mg/mL
• λ: 2 M in H₂O
• UV absorption: λ: 260 nm A_max: 0.01; λ: 280 nm A_max: 0.01
• suitability: in accordance for electrophoresis of RNA in agarose gels
• storage temp.: room temp
• SMILES string: [H]C([H])=O
• InChI: 1S/CH2O/c1-2/h1H2
• InChI key: WSFSSNUMVMOOMR-UHFFFAOYSA-N

Description

General description

Formaldehyde is a molecular biology reagent that denaturates RNA during electrophoresis when incorporated in agarose gels.

Application

Suitable for

• denaturation and separation of RNA molecules
• anti-bacterial treatment
• fixation of cells

Packaging

250, 250 mL

1, 2.5 L

Caution

may contain precipitate (paraformaldehyde)

Principle

Formaldehyde disrupts the secondary structure of RNA and allows separation and identification of RNA species based on charge migration.

Safety Information

• Pictograms: GHS02,GHS05,GHS06,GHS08
• Signal Word: Danger
• Hazard Statements: H226 - H301 + H311 - H314 - H317 - H330 - H335 - H341 - H350 - H370
• Precautionary Statements: P201 - P210 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 2 Inhalation - Acute Tox. 3 Dermal - Acute Tox. 3 Oral - Carc. 1B - Eye Dam. 1 - Flam. Liq. 3 - Muta. 2 - Skin Corr. 1B - Skin Sens. 1 - STOT SE 1 - STOT SE 3
• Target Organs: Respiratory system
• Storage Class Code: 3 - Flammable liquids
• WGK: WGK 3
• Flash Point(F): 133.0 °F - closed cup
• Flash Point(C): 56.11 °C - closed cup
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves