Merck
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51536C

SAFC

RPMI-1640 Medium

with 2.05 mM L-glutamine, with 25mM HEPES, liquid, sterile-filtered, suitable for cell culture

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MDL number:
MFCD00217820
NACRES:
NA.75

description

for research or for further manufacturing use

Quality Level

500

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | hybridoma: suitable
cell culture | mammalian: suitable

components

HEPES: 5958 mg/L
L-glutamine: 300 mg/L
NaHCO3: 2000 mg/L
phenol red: 5.310 mg/L

shipped in

ambient

storage temp.

2-8°C

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51536C

RPMI-1640 Medium

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form

liquid

form

powder

form

liquid

form

powder

technique(s)

cell culture | hybridoma: suitable, cell culture | mammalian: suitable

technique(s)

cell culture | hybridoma: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

components

HEPES: 5958 mg/L
L-glutamine: 300 mg/L
NaHCO3: 2000 mg/L
phenol red: 5.310 mg/L

components

phenol red: 0.0053 g/L
L-glutamine: 0.3 g/L
HEPES: 3.5745 g/L
NaHCO3: no
glucose: 4.5 g/L (Dextro)

components

NaHCO3: 2 g/L
phenol red: no
glucose: 2 g/L (Dextro)
L-glutamine: 0.3 g/L

components

L-glutamine: yes
NaHCO3: no
phenol red: yes
HEPES: 25 mM
sodium pyruvate: no

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

-

storage temp.

2-8°C

General description

RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage dependent.
RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage-dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells. This medium is suitable for culturing human normal and neoplastic leukocytes.

Application

RPMI-1640 Medium has been used to incubate dissected lung tissue.

supplement

Product No.
Description
Pricing

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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