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Ham′s Nutrient Mixture F12


Nutrient Mixture F12 Ham’s
MDL number:

Quality Level


for research or for further manufacturing use
with 1.0 mM L-glutamine






for further manufacturing use


cell culture | mammalian: suitable


phenol red: 1.274 mg/L
NaHCO3: 1176 mg/L
sodium pyruvate: 110.1 mg/L
L-glutamine: 146.2 mg/L

shipped in


storage temp.


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Ham′s Nutrient Mixture F12 was originally developed for the serum-free clonal growth of Chinese Hamster Ovary (CHO) cells, lung cells, and mouse L cells. It is frequently used with dialyzed serum, hormones, selenium, and other supplements for serum-free cultures. It is the medium of choice for supporting the growth of cells of rodent origin, particularly rabbbit and rat, and has been proven to be an excellent cloning medium for myeloma and hybridoma cells.

Storage Class Code

12 - Non Combustible Liquids



Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

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Certificate of Origin

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