Merck
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70425

Sigma-Aldrich

Atto 590

BioReagent, suitable for fluorescence, ≥90% (HPLC)

Empirical Formula (Hill Notation):
C37H39ClN2O9
Molecular Weight:
691.17
MDL number:
NACRES:
NA.32

product line

BioReagent

assay

≥90% (HPLC)

form

powder

manufacturer/tradename

ATTO-TEC GmbH

λ

in ethanol (with 0.1% trifluoroacetic acid)

UV absorption

λ: 595-601 nm Amax

suitability

suitable for fluorescence

storage temp.

−20°C

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Application

Atto fluorescent labels are designed for high sensitivity applications, including single-molecule detection. Atto labels have rigid structures that do not show any cis-trans isomerization. Thus these labels display exceptional intensity with minimal spectral shift on conjugation.
Atto 590 shows a molar extinction of 120,000 and QY of 80% in water (90% in ethanol). Decay time is 3.7 ns.
Employed in fluorescence energy transfer, FRET, microscopy.

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

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Certificate of Origin

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Matthew Antonik et al.
The journal of physical chemistry. B, 110(13), 6970-6978 (2006-03-31)
We establish a probability distribution analysis (PDA) method for the analysis of fluorescence resonance energy transfer (FRET) signals to determine with high precision the originating value of a shot-noise-limited signal distribution. PDA theoretical distributions are calculated explicitly including crosstalk, stochastic
D Wildanger et al.
Journal of microscopy, 236(1), 35-43 (2009-09-24)
The advent of supercontinuum laser sources has enabled the implementation of compact and tunable stimulated emission depletion fluorescence microscopes for imaging far below the diffraction barrier. Here we report on an enhanced version of this approach displaying an all-physics based
Uffe V Schneider et al.
Nucleic acids research, 38(13), 4394-4403 (2010-03-27)
Twisted intercalating nucleic acid (TINA) is a novel intercalator and stabilizer of Hoogsteen type parallel triplex formations (PT). Specific design rules for position of TINA in triplex forming oligonucleotides (TFOs) have not previously been presented. We describe a complete collection
Subunit rotation in a single F0F1-ATP synthase in a living bacterium monitored by FRET.
Seyfert, K.,et al.
Proceedings of SPIE, 7905, 79050K-79050K (2011)
Direct observation of the nanoscale dynamics of membrane lipids in a living cell. Nature.
Eggeling, C.; et al.
Nature, 457, 1159-1162 (2009)

Articles

Fluorescence Lifetime Measurement

Fluorescence lifetime measurement is advantageous over intensity-based measurements. Applications include fluorescence lifetime assays, sensing and FLI.

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