Merck
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71386

Sigma-Aldrich

Sodium chloride solution

BioUltra, for molecular biology, ~5 M in H2O

Synonym(s):
Halite, NaCl, sodium chloride
Linear Formula:
NaCl
CAS Number:
Molecular Weight:
58.44
Beilstein:
3534976
MDL number:
PubChem Substance ID:
NACRES:
NA.25

grade

for molecular biology

Quality Level

product line

BioUltra

form

liquid

concentration

~5 M in H2O

impurities

DNases, none detected
RNases, none detected
insoluble matter, passes filter test
phosphatases, none detected
proteases, none detected

refractive index

n20/D 1.378

pH

5.0-7.0 (25 °C)

density

1.186 g/mL (5 M in water)

cation traces

Al: ≤1 mg/kg
Ba: ≤1 mg/kg
Bi: ≤1 mg/kg
Ca: ≤5 mg/kg
Cd: ≤1 mg/kg
Co: ≤1 mg/kg
Cr: ≤1 mg/kg
Cu: ≤1 mg/kg
Fe: ≤1 mg/kg
K: ≤20 mg/kg
Li: ≤1 mg/kg
Mg: ≤1 mg/kg
Mn: ≤1 mg/kg
Mo: ≤1 mg/kg
Ni: ≤1 mg/kg
Pb: ≤1 mg/kg
Sr: ≤1 mg/kg
Zn: ≤1 mg/kg

λ

neat

UV absorption

λ: 260 nm Amax: 0.02
λ: 280 nm Amax: 0.01

SMILES string

[Na+].[Cl-]

InChI

1S/ClH.Na/h1H;/q;+1/p-1

InChI key

FAPWRFPIFSIZLT-UHFFFAOYSA-M

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General description

Sodium chloride is a vital nutrient that aids normal body functions in humans and animals. This chemical is commonly found in nature with a wide variety of biochemical applications, like intravenous fluids and density gradients. It is also used as a diluent to increase ionic strength in buffers or culture media. Sodium chloride is crucial for maintaining plasma osmolality and the extracellular fluid volume of the body. Excess NaCl leads to increased blood pressure, which has implications for cardiovascular and renal diseases. Sterile NaCl is a component of eye drop solutions. Traditionally, it has been used in high concentrations to preserve food since most bacteria cannot grow in high-salt conditions.

Application

Sodium chloride solution has been used as a component of:
  • polyethylene glycol (PEG) buffer for the purification of double-tagged products
  • NT2 buffer for the preparation of antibody-coated Protein A Sepharose (PAS) beads and immunoprecipitation of ribonucleoproteins (RNPs)
  • chilled lysis buffer for the isolation of single nuclei from mice kidney

Features and Benefits

  • Geologically stable
  • Suitable for purification, precipitation, and crystallization

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

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Certificate of Origin

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