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Atto 590 NHS ester

BioReagent, suitable for fluorescence, ≥90.0% (degree of coupling)

Empirical Formula (Hill Notation):
CAS Number:
Molecular Weight:
MDL number:

Quality Level

product line



≥90.0% (HPLC)
≥90.0% (degree of coupling)






in ethanol (with 0,1% trifluoroacetic acid)

UV absorption

λ: 597-663 nm Amax


suitable for fluorescence

detection method


storage temp.


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General description

Atto 590 is a novel fluorescent label belonging to the class of Rhodamine dyes. The dye is designed for application in the area of life science, e.g. labeling of DNA, RNA or proteins. The Atto dyes are a series of fluorescent dyes that provide all the crucial properties required for modern fluorescent technologies, such as fluorescence microscopy, flow-cytometry, fluorescence in situ hybridization (FISH), receptor binding assays or enzyme assays. The dye is highly suitable for single-molecule detection applications and high-resolution microscopy. The NHS-esters are used in common conjugation protocols.
Atto 590 is a novel fluorescent label belonging to the class of Rhodamine dyes, which has an absorption maximum of 594 nm and an emission maximum of 624 nm. Atto 590-N-hydroxysuccinimide (NHS) is membrane permeable.


Molar absorption 120,000 1/M cm, abs: 593 nm, em: 620 nm, QY=0.93, Tfl 4.0 ns (unpublished data)
Atto 590 NHS ester may be suitable for use in site-specific labeling of human embryonic kidney (HEK293T) cell lysates for western blotting, fluorescence, and widefield microscopy studies.

Features and Benefits

  • Absorption maxima cover a range from 590 to 620 nm.
  • Structural properties support extraordinary photostability.
  • High fluorescence quantum yield and high thermal stability.
  • The fluorescence characteristics of the dye are quite insensitive to environmental changes (e.g., pH changes). This holds for both excitation and emission wavelengths and for the decay time of their fluorescence emission.
  • Compatible with common labeling procedures for proteins and amino-functionalized oligonucleotides.

Other Notes

Study of multistep energy transfer in a single photonic wire by FRET; employed in fluorescence energy transfer, FRET, scanning microscopy.

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Storage Class Code

13 - Non Combustible Solids



Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

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Certificate of Origin

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Handbook of fluorescence spectroscopy and imaging: from ensemble to single molecules null
Y. Eberstein et al.
J. Chem. Phys. , B 108, 93-93 (2004)
D Wildanger et al.
Journal of microscopy, 236(1), 35-43 (2009-09-24)
The advent of supercontinuum laser sources has enabled the implementation of compact and tunable stimulated emission depletion fluorescence microscopes for imaging far below the diffraction barrier. Here we report on an enhanced version of this approach displaying an all-physics based
Martin Baumdick et al.
Nature communications, 9(1), 3847-3847 (2018-09-23)
Epidermal growth factor receptor (EGFR) activation by growth factors (GFs) relies on dimerization and allosteric activation of its intrinsic kinase activity, resulting in trans-phosphorylation of tyrosines on its C-terminal tail. While structural and biochemical studies identified this EGF-induced allosteric activation
Uffe V Schneider et al.
Nucleic acids research, 38(13), 4394-4403 (2010-03-27)
Twisted intercalating nucleic acid (TINA) is a novel intercalator and stabilizer of Hoogsteen type parallel triplex formations (PT). Specific design rules for position of TINA in triplex forming oligonucleotides (TFOs) have not previously been presented. We describe a complete collection


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