93482

Phenylmethanesulfonyl fluoride solution

~0.1 M in ethanol (T)

• Synonym(s): Benzylsulfonyl fluoride, PMSF
• CAS Number: 329-98-6
• Beilstein: 2088311
• MDL number: MFCD00007424
• PubChem Substance ID: 57653563
• NACRES: NA.77

Properties

• biological source: microbial; synthetic
• Quality Level: 200
• form: liquid
• concentration: ~0.1 M in ethanol (T)
• density: 0.797 g/mL at 20 °C
• storage temp.: 2-8°C
• SMILES string: FS(=O)(=O)Cc1ccccc1
• InChI: 1S/C7H7FO2S/c8-11(9,10)6-7-4-2-1-3-5-7/h1-5H,6H2
• InChI key: YBYRMVIVWMBXKQ-UHFFFAOYSA-N

Description

Application

Phenylmethanesulfonyl fluoride solution has been used as a component in the cell lysate buffer in breast adenocarcinoma cell line, fibrosarcoma cell line and D5 hindbrain and spinal cord neural progenitors.

Packaging

50, 250 mL

Biochem/physiol Actions

Administration of PMSF produces analgesia unrelated to its anticholinesterase effect, and prolongs the analagesic effect of centrally administered β-endorphin.

Phenylmethanesulfonyl fluoride (PMSF) is a serine protease inhibitor. It binds covalently to active site residue of proteases and prevents proteolytic activity. PMSF is used in cell lysate buffers during protein purification to prevent target protein degradation. PMSF effectively inhibits the naloxone-precipitated withdrawal jumping.

Safety Information

• Pictograms: GHS02,GHS07
• Signal Word: Danger
• Hazard Statements: H225 - H315 - H319
• Precautionary Statements: P210 - P233 - P240 - P241 - P303 + P361 + P353 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Flam. Liq. 2 - Skin Irrit. 2
• Storage Class Code: 3 - Flammable liquids
• WGK: WGK 2
• Flash Point(F): 53.6 °F - closed cup
• Flash Point(C): 12 °C - closed cup
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves