10 - Combustible liquids
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
This reagent, as supplied and stored at 2-8°C, will be stable for four years from the date of manufacturing. Freezing is not recommended, because it may cause precipitation of the inhibitors.
In most circumstannces, the product is used at a 100-fold dilution. This will inhibit the phosphatases in 5 mg of protein per mL of lysate.
Since this solution is supplied in DMSO, it may solidify (melting point of DMSO is 16-19 deg C). Simply allow it to melt before use. This will not affect the product.
Cantharidin (Product Code C7632) inhibits protein phosphatase 2A (PP-2A). Bromotetramisole (Product Code B8132) inhibits alkaline phosphatases. Microcystin LR (Product Code M2912) inhibits protein phosphatases 1 and 2A (PP-1 and PP-2A). The concentrations of these inhibitors are considered proprietary.
As far as we know, the phosphatases in mammalian cells also appear in yeast cells. Thus both cocktails, P5726 (Phosphatase cocktail II and P2850 (Phosphatase Inhibitor cocktail I), may be needed with yeast extracts.
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Ask a Scientist here.
The use of phosphatase inhibitors is critical for all types of phosphorylation studies. Compare phosphatase inhibitor cocktails to select the best one for your research.