Merck
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A1602

Sigma-Aldrich

Amyloglucosidase from Aspergillus niger

ammonium sulfate suspension, ≥40 units/mg protein

Synonym(s):
Glucoamylase, 1,4-α-D-Glucan glucohydrolase, Exo-1,4-α-glucosidase
CAS Number:
Enzyme Commission number:
MDL number:
NACRES:
NA.54

Quality Level

form

ammonium sulfate suspension

specific activity

≥40 units/mg protein

shipped in

wet ice

storage temp.

2-8°C

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General description

Amyloglucosidase, a disaccharide enzyme is a member of the alpha-glucosidase type. It is produced by several species of Aspergillus genus.

Application

Amyloglucosidase from Aspergillus niger has been used to liberate free glucose in the Belgica antarctica larva homogenate. It has also been used in digestive enzyme mixture for starch digestion.
Amyloglucosidase from Aspergillus niger is used to hydrolyze α-D-glucosides. It may be used in the brewing of beer and in the production of bread and juices. Amyloglucosidase, from Sigma, has been used to hydrolyze glycogen into glucose monomers in order to study lipid accumulation in skeletal muscle .

Biochem/physiol Actions

Amyloglucosidase from Aspergillus niger is capable of hydrolyzing the α-D-(1-4), the α-D-(1-6), and the α-D-(1-3) glucosidic bonds of oligosaccharides .

Unit Definition

One unit will liberate 1.0 mg of glucose from starch in 3 min at pH 4.5 at 55 °C.

Physical form

Suspension in 3.2 M (NH4)2SO4 solution, pH approx. 6.0

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

Survival and energetic costs of repeated cold exposure in the Antarctic midge, Belgica antarctica: a comparison between frozen and supercooled larvae
Teets NM, et al.
The Journal of Experimental Biology, 214(5), 806-814 (2011)
A method for routine measurements of total sugar and starch content in woody plant tissues
Chow PS and Landhausser SM
Tree physiology, 24(10), 1129-1136 (2004)
Biodiversity: New Leads for the Pharmaceutical and Agrochemical Industries, 183-183 (2000)
Jane Usher et al.
Applied and environmental microbiology, 75(13), 4573-4579 (2009-05-12)
Yeasts used in the production of lagers contain complex allopolyploid genomes, resulting from the fusion of two different yeast species closely related to Saccharomyces cerevisiae and Saccharomyces bayanus. Recombination between the homoeologous chromosomes has generated a number of hybrid chromosomes.
Hugh S Winwood-Smith et al.
American journal of physiology. Regulatory, integrative and comparative physiology, 313(4), R347-R356 (2017-07-14)
Long-term studies have found that low-carbohydrate diets are more effective for weight loss than calorie-restricted diets in the short term but equally or only marginally more effective in the long term. Low-carbohydrate diets have been linked to reduced glycogen stores

Protocols

Enzymatic Assay of Amyloglucosidase (EC 3.2.1.3)

This procedure may be used for the determination of Amyloglucosidase activity using starch as the substrate.

Related Content

How to Work with Enzymes Supplied as Ammonium Sulfate Suspensions

Instructions for working with enzymes supplied as ammonium sulfate suspensions

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