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Bovine Serum Albumin

chromatographically purified, New Zealand origin, low endotoxin, suitable for cell culture, pH 7, ≥98%

Albumin bovine serum, BSA, Bovine albumin
CAS Number:
EC Number:
MDL number:

biological source


Quality Level

product line





lyophilized powder

mol wt

~66 kDa

purified by



poly bottle of


New Zealand origin


cell culture | mammalian: suitable


≤1 EU/mg endotoxin




water: soluble (40 mg/ml)

UniProt accession no.

storage temp.


Gene Information

bovine ... ALB(280717)

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General description

Bovine serum albumin (BSA) is a 66 kDa globular protein crucial for transporting molecules like fatty acids, drugs and hormones from the bloodstream. BSA is a drug carrier for bioactive compounds and has wide clinical applications. BSA has extensive usage in biological research including cell culture, molecular biology, protein biochemistry, and detection techniques. It has wide pharmaceutical applications due to ease of production and interaction flexibility.


Bovine serum albumin has been used:
  • as a standard in bradford protein estimation method in yeast cells
  • in elispot assay of blood cells
  • as a blocking agent in immunohistochemical studies of transplant
  • as a blocking agent in western blotting of human gastric cancer cell lines

Biochem/physiol Actions

Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.

Features and Benefits

  • Tested for use in cell culture
  • Low endotoxin

Preparation Note

Prepared by salt fractionation, ion exchange, and gel filtration chromatography.
Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.

Storage Class Code

11 - Combustible Solids



Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

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Bidirectional juxtacrine ephrinB2/Ephs signaling promotes angiogenesis of ECs and maintains self-renewal of MSCs
Cao C, et al.
Biomaterials, 172(1-13), 1-13 (2018)
Review on albumin as drug carrier in treating different diseases and disorders
Naveen R, et al.
Pharmacia Sinica, 7(1), 11-15 (2017)
Cloning, characterization and expression of an exo-1, 3-beta-glucanase gene from the antagonistic yeast, Pichia guilliermondii strain M8 against grey mold on apples
Zhang D, et al.
Biological Control, 59(2), 284-293 (2011)
Quantification of self-recognition in multiple sclerosis by single-cell analysis of cytokine production
Pelfrey CM, et al.
Journal of Immunology, 165(3), 1641-1651 (2000)
Gene regulatory network construction identified NFYA as a diffuse subtype-specific prognostic factor in gastric cancer
Cao B, et al.
International Journal of Oncology, 53(5), 1857-1868 (2018)


Albumin in Cell Culture

This page segues to comprehensive insights on how serum albumin and other important cell culture components affect the performance of serum-free cell culture systems used for biomanufacturing heterologous proteins including monoclonal antibodies. The page introduces the in vitro chemistry and biochemistry of albumin. These insights may also be applied to ex vivo and tissue engineering applications.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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